The Insect BAC Library Initiative: A Fundamental Resource for the Study of Evolution and Development

0208278 romero-seversondr。Romero-Severson等人根据五个昆虫类群（Tribolium castaneum, Nassonia vitripennis, Oncopeltus fasciatus， Thermobia domestica和Schistocerca Americana）的系统发育地位、科学重要性和现有基因组资源的稀缺性，提出了构建BAC文库的建议。这些分类群代表了昆虫目鞘翅目（甲虫）、膜翅目（黄蜂和蜜蜂）、半翅目（真虫）、飞尾目（银鱼）和直翅目（蚱蜢和蟋蟀）。结合双翅目昆虫黑腹果蝇和冈比亚按蚊的全基因组序列，这些文库将使科学界能够检验有关昆虫遗传学、基因组学、发育、生态学、系统学和进化的重要假设。这项工作将与普渡大学正在进行的昆虫基因组数据库的开发相吻合。克莱姆森大学基因组学研究所（CUGI）是BAC图书馆建设的分包商。在过去的五年中，中国农业大学生物技术中心（CUGI） BAC/EST资源中心构建了大部分用于农业基因组学的植物和真菌BAC文库。CUGI BAC/EST资源中心将提供昆虫库作为高密度杂交过滤器和克隆，并在成本回收的基础上用于学术项目。

0208278Romero-SeversonDr. Romero-Severson and colleagues propose the construction of BAC libraries for five insect taxa (Tribolium castaneum, Nassonia vitripennis, Oncopeltus fasciatus, Thermobia domestica and Schistocerca Americana) selected for their phylogenetic position, scientific importance and scarcity of existing genomic resources. These taxa represent the insect orders Coleoptera (beetles), Hymenoptera (wasps and bees), Hemiptera (true bugs), Thysanura (silverfish) and Orthoptera (grasshoppers and crickets).In combination with the whole genome sequences of the Dipteran insects Drosophila melanogaster and Anopheles gambiae, these libraries will enable the scientific community to test important hypotheses concerning insect genetics, genomics, development, ecology, systematics, and evolution. This effort will dovetail with the ongoing development of an insect genomics database at Purdue University. The Clemson University Genomics Institute (CUGI) is the subcontractor for the BAC library construction. Over the past five years, the CUGI BAC/EST Resource Center has constructed most of the plant and fungal BAC libraries used in agricultural genomics. The CUGI BAC/EST Resource Center will supply the insect libraries as high-density hybridization filters and clones on a cost recovery basis for academic programs.