Analysis of differential functional capacity and origins of memory B-lymphocyte subsets

记忆 B 淋巴细胞亚群的差异功能能力和起源分析

• 批准号: 168822126
• 负责人: Dr. Florian Weisel
• 金额: --
• 依托单位: Department of Immunology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/168822126?language=en
• 关键词: Analysis; differential; functional; capacity; origins

Immunological memory is a defining feature of the adaptive immune response. Very recently it was shown in the laboratory of Dr. M. Shlomchik that memory B-lymphocytes are comprised of clear phenotypic subsets that can be identified by specific expression of the cell-surface markers CD80, PD-L2 and CD73. By using NP-specific murine memory B cell systems, which provide a large number of antigen-specific memory B cells after immunization, I will further characterize these newly described subsets in the proposed fellowship. Main objectives are to determine their origins and to test whether memory B cell subsets have differential functional capacity. Experiments will be performed to address whether type and degree of stimulation influences the subset type of memory B cells generated. Subsets will be purified to identify proliferative and differentiative capacity in vitro and in vivo. These subsets will be further dissected using gene expression profiling, RT-qPCR, and FACS, comparing the subsets to each other and to naïve cells in order to obtain insight into differential function. The delineation of such memory B cell subsets should provide important insight into how secondary B cell responses function.

免疫记忆是适应性免疫反应的一个重要特征。最近，它在M博士的实验室里被展示出来。Shlomchik认为，记忆B淋巴细胞由明确的表型亚群组成，这些亚群可以通过细胞表面标志物CD 80、PD-L2和CD 73的特异性表达来鉴定。通过使用NP特异性小鼠记忆B细胞系统，免疫后提供大量抗原特异性记忆B细胞，我将进一步表征这些新描述的亚群在拟议的奖学金。主要目的是确定它们的起源，并测试记忆B细胞亚群是否具有不同的功能能力。将进行实验以解决刺激的类型和程度是否影响所产生的记忆B细胞的亚群类型。将纯化亚组以鉴定体外和体内的增殖和分化能力。将使用基因表达谱分析、RT-qPCR和FACS进一步解剖这些亚群，将亚群相互比较并与幼稚细胞比较，以获得对差异功能的了解。这种记忆B细胞亚群的描述应该为了解次级B细胞反应如何发挥作用提供重要的见解。