SGER: Physical fractionation of marine microbes for efficient mining and description of microbial diversity

PIS计划利用大规模浓缩和连续制备性分馏技术，将复杂的微生物组合分离成它们的组成种群。其目标是实现高度的浓缩，甚至是纯度，并具有足够的生物量产量，以便能够在不进行培养的情况下对单个微生物种群进行形态、生化和基因组分析。这项工作应导致开发更有效地挖掘复杂微生物群落中微生物多样性的方法，在这些群落中，许多组成种群不容易培养。如果成功，这项工作将使对未培养微生物的基因组、蛋白质组和生化同时分析成为可能。在不进行培养的情况下成功地提纯选定种群的大量生物量也将使自然同位素比率的分析能够提供线索，以了解这些种群的就地代谢过程。

The PIs plan to exploit large-scale concentration and serial, preparative fractionation techniques as a means to separate complex microbial assemblages into their component populations. The goal is to achieve high degrees of enrichment, or even purity, with a sufficient biomass yield to allow morphological, biochemical, and genomic analyses on individual microbial populations without cultivation. The work should result in the development of methods for more efficiently mining microbial diversity in complex microbial communities where many of the constituent populations are not readily cultivable. If successful, this work will make possible simultaneous analysis of the genome, proteome, and biochemistry of even uncultivated microbes. Successful purification of large amounts of biomass of selected populations without culturing will also allow analyses of natural isotopic ratios that could provide clues to the metabolic processes of those populations in situ.