PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
批准号:
3336613
负责人:
KENNETH C. INGHAM
金额:
$18.72万
依托单位国家:
美国
项目类别:
财政年份:
1978
资助国家:
美国
项目状态:
已结题
起止时间:
1978-01-01 至 1986-08-31
关键词:
activation product anticoagulants antiseptic sterilization antithrombin III antithrombins blood fractionation blood preservation blood proteins chemical structure function complement enzyme substrate complex fibrin fibrinogen fluorescence spectrometry heparin human tissue intermolecular interaction plasma plasmin polyethylenes protease inhibitor radiotracer solubility thermostability zymogens
中文摘要
这一建议描述了对选定的物理化学性质的研究
英文摘要
This proposal describes research into the physicochemical properties of selected
plasma proteins including antithrombin III, Cl-inactivator, alpha2-plasmin
inhibitor, and fibronectin, all of which have potential value for replacement
therapy in patients with either inherited or acquired deficiences. The program
will expedite the development of new or improved methods of isolating and
preserving these proteins in a form suitable for clinical evaluation. The
solubility of various proteins will be evaluated in the presence of polyethylene
glycol, an inert fractional precipitating agent, which is being developed as an
alternative to ethanol for large-scale fractionation. We will seek ways of
selectively manipulating the solubility of specific proteins, for example by
addition of substances with which they preferentially interact. Secondly, these
proteins will be examined with respect to their stability towards various forms
of stress, with particular emphasis on thermal stability, the objective being to
define conditions under which they can be pasteurized to diminish the risk of
transfusion hepatitis. Finally, we propose to develop a broad program of
investigation of protein-protein and protein-ligand interactions in human
plasma, with emphasis on the use of fluorescence spectroscopy and high pressure
liquid chromatography. Systems to be investigated include: (1) the interaction
of the above three protease inhibitors with the relevant proteases and zymogens,
(2) the interaction of fibronectin with fibrin(ogen), collagen, and heparin, (3)
the interaction between various fragments of these proteins. The results of
these studies, in addition to clarifying structure/function relationships, will
be applied to the development of fluorescent binding assays and improved
purification schemes based on affinity principles.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Core--Macromolecular analysis and interactions
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批准号:6644330
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项目类别:
-
资助金额:$13.59万
-
财政年份:2002
-
负责人:KENNETH C. INGHAM
-
依托单位:
SHARED CIRCULAR DICHROMETER/FLUOROMETER
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批准号:6291973
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项目类别:
-
资助金额:$11.0万
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财政年份:2001
-
负责人:KENNETH C. INGHAM
-
依托单位:
Core--Macromolecular analysis and interactions
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批准号:6504144
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项目类别:
-
资助金额:$13.59万
-
财政年份:1996
-
负责人:KENNETH C. INGHAM
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依托单位:
DOMAIN STRUCTURE OF PLATELET INTEGRIN IIB-IIIA
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批准号:3432702
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项目类别:
-
资助金额:$2.0万
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财政年份:1993
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负责人:KENNETH C. INGHAM
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依托单位:
DOMAIN STRUCTURE OF PLATELET INTEGRIN IIB-IIIA
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批准号:2291749
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项目类别:
-
资助金额:$2.0万
-
财政年份:1993
-
负责人:KENNETH C. INGHAM
-
依托单位:
DOMAIN STRUCTURE OF PLATELET INTEGRIN IIB-IIIA
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批准号:2291750
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项目类别:
-
资助金额:$2.0万
-
财政年份:1993
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负责人:KENNETH C. INGHAM
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依托单位:
NHLBI SHARED RESEARCH FACILITY FOR MOLECULAR BIOLOGY
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批准号:3003479
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项目类别:
-
资助金额:$24.6万
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财政年份:1987
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负责人:KENNETH C. INGHAM
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依托单位:
INTERACTIONS OF FIBRONECTIN MODULES
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批准号:2685278
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项目类别:
-
资助金额:$23.74万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
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批准号:3336616
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项目类别:
-
资助金额:$23.22万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
-
批准号:3336615
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项目类别:
-
资助金额:$13.96万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
DOMAIN STRUCTURE AND MATRIX INTERACTIONS OF FIBRONECTIN
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批准号:3336612
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项目类别:
-
资助金额:$20.09万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
INTERACTIONS OF FIBRONECTIN MODULES
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批准号:6182881
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项目类别:
-
资助金额:$25.18万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
-
批准号:3336618
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项目类别:
-
资助金额:$21.56万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
-
批准号:3336617
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项目类别:
-
资助金额:$22.93万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
PHYSICAL CHEMISTRY OF THERAPEUTIC PLASMA PROTEINS
-
批准号:3336614
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项目类别:
-
资助金额:$8.99万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
INTERACTIONS OF FIBRONECTIN MODULES
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批准号:2028024
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项目类别:
-
资助金额:$23.05万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
DOMAIN STRUCTURE AND MATRIX INTERACTIONS OF FIBRONECTIN
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批准号:2215493
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项目类别:
-
资助金额:$22.07万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
INTERACTIONS OF FIBRONECTIN MODULES
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批准号:2901030
-
项目类别:
-
资助金额:$24.45万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
DOMAIN STRUCTURE AND MATRIX INTERACTIONS OF FIBRONECTIN
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批准号:2215492
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项目类别:
-
资助金额:$21.69万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
DOMAIN STRUCTURE AND MATRIX INTERACTIONS OF FIBRONECTIN
-
批准号:2215494
-
项目类别:
-
资助金额:$23.17万
-
财政年份:1978
-
负责人:KENNETH C. INGHAM
-
依托单位:
海外基金