Carboxysomes:The Role of Microcompartmentalization in Bacterial CO2 Fixation

羧基体:微区室化在细菌 CO2 固定中的作用

基本信息

  • 批准号:
    0444568
  • 负责人:
  • 金额:
    $ 42.14万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-02-15 至 2008-05-31
  • 项目状态:
    已结题

项目摘要

All cyanobacteria and many chemoautotrophic bacteria contain polyhedral inclusion bodies called carboxysomes. These microcompartments, which consist entirely of protein and are filled with ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO), are thought to be part of a carbon concentrating mechanism (CCM) that serves to enhance the CO2 fixation efficiency in organisms that contain them. Although the molecular mechanism by which the carboxysome provides a catalytic enhancement for RubisCO is not certain, it has long been a tenet of the CCM hypothesis that a carboxysomal carbonic anhydrase (CA) rapidly converts cytoplasmic HCO3 - into CO2, the substrate for RubisCO. Recent work from this laboratory established that a protein component of the carboxysomal shell is a novel CA that we have term EPSCOR-CA. Its localization in the carboxysomal shell suggests that carboxysomes function via a mechanism that is more complex than that predicted by the standard CCM model and might indicate an expanded role for the microcompartments in autotrophic carbon assimilation. Biochemical and molecular biological approaches will be combined to elucidate structural features of the carboxysome that form the basis for its function, further characterize the newly discovered carboxysomal CA biochemically and identify its interactions with other proteins in the shell. The obligate chemoautotrophic bacterium Halothiobacillus neapolitanus will serve as a model system for these studies since a) homogeneous preparations of carboxysomes can be isolated in large quantities from the organism, b) its carboxysome operon has been cloned, characterized and expressed in Escherichia coli, c) a substantial number of carboxysome mutants have been created in H. neapolitanus, d) the bacterium can be genetically manipulated, e) gene clusters homologous to the putative operon encoding H. neapolitanus carboxysomes have been observed in both cyanobacteria and other species of chemoautotrophs. Broader Impacts Given the large contribution of carboxysome-containing organisms towards CO2 fixation and the impact of their metabolism on the global carbon cycle, one broader implication of the work proposed will be the elucidation of one of the most basic and central biochemical processes that control the carbon cycle and influence weather and climate. The PI will be directly involved in conducting key experiments and will be the mentor for graduate and undergraduate student research in various aspects of the research. Students participating in these projects will gain experience in biochemical and molecular biological research techniques.
所有的蓝藻和许多化能自养细菌都含有多角形的包含体,称为羧化体。这些微区室完全由蛋白质组成,充满了核酮糖1,5-二磷酸羧化酶/加氧酶(RubisCO),被认为是碳浓缩机制(CCM)的一部分,用于提高含有它们的生物体的CO2固定效率。虽然羧基体提供RubisCO催化增强的分子机制尚不确定,但长期以来CCM假说的一个原则是羧基体碳酸酐酶(CA)快速将细胞质HCO 3-转化为CO2,RubisCO的底物。该实验室最近的工作确定,羧基体壳的蛋白质组分是一种新的CA,我们称之为EPSCOR-CA。它的本地化carboxysomal壳表明,carboxysomes功能通过一种机制,这是更复杂的比标准CCM模型预测,并可能表明扩大的作用,在自养碳同化的微隔间。 生物化学和分子生物学的方法将结合起来,以阐明其功能的基础上形成的羧基体的结构特征,进一步表征新发现的carboxysomal CA生化和识别其与其他蛋白质的相互作用在壳。专性化能自养细菌那不勒斯盐硫杆菌将作为这些研究的模型系统,因为a)可以从生物体中大量分离羧基体的均质制备物,B)其羧基体操纵子已经在大肠杆菌中克隆、表征和表达,c)在H. neapolitanus,d)该细菌可以被遗传操纵,e)与编码H.在蓝细菌和其他化学自养生物物种中都观察到了那不勒斯羧化酶体。 更广泛的影响 考虑到含碳生物体对二氧化碳固定的巨大贡献及其代谢对全球碳循环的影响,拟议工作的一个更广泛的意义将是阐明控制碳循环并影响天气和气候的最基本和最重要的生化过程之一。 PI将直接参与进行关键实验,并将在研究的各个方面为研究生和本科生的研究导师。参加这些项目的学生将获得生物化学和分子生物学研究技术的经验。

项目成果

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Gordon Cannon其他文献

Gordon Cannon的其他文献

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{{ truncateString('Gordon Cannon', 18)}}的其他基金

Collaborative Research: Structural, Functional, and Ecological Characterization of the Prochlorococcus Carboxysome, the Ocean's Primary Molecular Module for Carbon Fixation
合作研究:原绿球菌羧基体(海洋固碳的主要分子模块)的结构、功能和生态特征
  • 批准号:
    0851070
  • 财政年份:
    2009
  • 资助金额:
    $ 42.14万
  • 项目类别:
    Standard Grant
Towards a Structure Based Mechanism for the Function of the Carboxysome, the Prototype Bacterial Organelle
探索基于羧基体(原型细菌细胞器)功能的结构机制
  • 批准号:
    0818680
  • 财政年份:
    2008
  • 资助金额:
    $ 42.14万
  • 项目类别:
    Standard Grant
Center for Ocean Sciences Education Excellence: Central Gulf of Mexico (COSEE:CGOM)
海洋科学教育卓越中心:墨西哥湾中部(COSEE:CGOM)
  • 批准号:
    0528597
  • 财政年份:
    2005
  • 资助金额:
    $ 42.14万
  • 项目类别:
    Continuing Grant

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