CAREER: Sulfur Oxidation in Chlorobium tepidum, a Model Phototrophic Bacterium

职业：温热绿菌（一种模型光养细菌）中的硫氧化

• 批准号: 0447649
• 负责人: Thomas Hanson
• 金额: $ 84.95万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0447649&HistoricalAwards=false
• 关键词: CAREER; Sulfur; Oxidation; Chlorobium; tepidum

This career development plan emphasizes genome-enabled studies of microbial physiology in a non-traditional microbial model system. The research component will provide a deeper understanding of how green sulfur bacteria (Chlorobiaceae), a widespread group of anoxygenic phototrophic bacteria, oxidize reduced sulfur compounds under anaerobic conditions. Anaerobic sulfur oxidation consumes reduced sulfur compounds, which are toxic to humans and other organisms. Anaerobic sulfur oxidation is a critical part of energy generation pathways in green sulfur bacteria, but it is one of the most poorly understood functions carried out by the Chlorobiaceae. The specific genetic components required for sulfur oxidation will be identified by a molecular genetic approach in Chlorobium tepidum, a recognized model system for the Chlorobiaceae. Characterization of mutants lacking sulfur oxidation enzymes coupled with biochemical studies will detail the contributions of these enzymes to the physiology of C. tepidum. This project will develop and disseminate genetic tools that will assist in analysis of the nine other Chlorobiaceae genomes currently being sequenced.Broader impacts: An outreach component will produce a science curriculum module that satisfies national educational standards. The module will use Winogradsky columns to illustrate the dynamic interaction between microbes and their environment. One unique aspect of the project is a strong integration with the research program. This will be accomplished by using student-constructed Winogradsky columns as sources of sulfur dependent phototrophs, which will be brought into axenic culture in the PI's laboratory. These cultures will be analyzed to assess the diversity and distribution of key sulfur oxidation genes identified by the research component. Culture properties, histories, images and data will be placed on a Web site for access by participating classrooms and the general public to complete an interactive loop between university research and K-12 education.

该职业发展计划强调在非传统微生物模型系统中进行微生物生理学的基因组研究。研究部分将提供一个更深入的了解如何绿色硫细菌（绿藻科），一个广泛的群体的厌氧光合细菌，氧化还原硫化合物在厌氧条件下。厌氧硫氧化消耗还原的硫化合物，其对人类和其他生物体有毒。厌氧硫氧化是绿色硫细菌能量产生途径的重要组成部分，但它是由绿藻科进行的最知之甚少的功能之一。硫氧化所需的特定遗传组分将通过分子遗传学方法在Chlorobium tepidum中鉴定，Chlorobiaceae的公认模式系统。缺乏硫氧化酶的突变体的特性与生物化学研究相结合，将详细说明这些酶对C。单调乏味。该项目将开发和传播遗传工具，协助分析目前正在测序的其他九个绿藻科基因组。更广泛的影响：外展部分将制作满足国家教育标准的科学课程模块。该模块将使用Winogradsky列来说明微生物和它们的环境之间的动态相互作用。该项目的一个独特之处是与研究计划的紧密结合。这将通过使用学生构建的Winogradsky柱作为硫依赖性光养生物的来源来实现，该硫依赖性光养生物将在PI的实验室中进行纯培养。将对这些培养物进行分析，以评估研究组成部分确定的关键硫氧化基因的多样性和分布。文化财产、历史、图像和数据将放在一个网站上，供参与的教室和公众访问，以完成大学研究和K-12教育之间的互动循环。