CAREER: Molecular Architecture in Peptide-Functionalized Biomaterials from NMR Spectroscopy

俄克拉荷马大学的查尔斯·赖斯得到了实验物理化学计划的支持，他使用高分辨率魔角旋转和多维核磁共振(核磁共振)光谱技术来研究与细胞在二维表面上的黏附和生长有关的特定多肽的结构和动力学。硅胶表面的多肽涂层将作为硬组织植入物的模型进行研究，多肽修饰的丙烯酰胺聚合物形成的生物活性水凝胶将被研究以模拟组织支架材料。已知含有精氨酸-甘氨酸-天冬氨酸(RGD)的多肽用作生物材料涂层时，可以减少炎症、疼痛、免疫反应和排斥反应。将对各种RGD涂层二氧化硅和RGD水凝胶样品进行表征，首先目的是了解RGD呈现的规则，最终目标是创造新的硅基和水凝胶生物材料。材料科学和生物化学已经融合在一起，产生了重大影响，特别是在组织植入物和设备方面的进步。化学分析和核磁共振波谱可以使人们对生物分子结构有新的了解，这些信息可能导致新的生物材料范式。这个项目包括一个旨在让高中生、本科生和研究生接触尖端研究的教育计划。这一努力将包括一个面向高中生和他们的老师的外展计划，涉及生物材料的合成和评估。此外，PI还将在本科生和研究生层面开发新的课程。

Charles Rice of the University of Oklahoma is supported by the Experimental Physical Chemistry Program to use high-resolution magic angle spinning and multidimensional nuclear magnetic resonance (NMR) spectroscopy techniques to examine the structure and dynamics of specific peptides involved in cell adhesion and growth on two-dimensional surfaces. Peptide coatings on silica will be studied as a model of hard-tissue implants, and bioactive hydrogels formed by acrylamide polymers that have been modified with peptides will be studied to simulate tissue-scaffold materials. Peptides that contain the arginine-glycine-aspartate (RGD) are known to reduce inflammation, pain, immune response, and rejection when used as a biomaterial coating. A variety of RGD-coated silica and RGD-hydrogel samples will be characterized, first with the aim of understanding the rules of RGD presentation, with the ultimate goal of creating new silica-based and hydrogel biomaterials.Materials science and biological chemistry have converged with significant impacts, particularly with respect to advances in tissue implants and devices. Chemical analysis and NMR spectroscopy can enable new understanding of biomolecular structure, information that may lead to new biomaterial paradigms. This project includes an education plan that aims to expose high school, undergraduate, and graduate students to cutting edge research. This effort will include an outreach program to high school students and their teachers, involving the synthesis and evaluation of biomaterials. As well, the PI will develop new curricula at the undergraduate and graduate levels.