CAREER: Molecular Architecture in Peptide-Functionalized Biomaterials from NMR Spectroscopy

职业：通过核磁共振波谱研究肽功能化生物材料的分子结构

• 批准号: 0449622
• 负责人: Charles Rice
• 金额: $ 55.56万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0449622&HistoricalAwards=false
• 关键词: CAREER; Molecular; Architecture; Peptide; Functionalized

Charles Rice of the University of Oklahoma is supported by the Experimental Physical Chemistry Program to use high-resolution magic angle spinning and multidimensional nuclear magnetic resonance (NMR) spectroscopy techniques to examine the structure and dynamics of specific peptides involved in cell adhesion and growth on two-dimensional surfaces. Peptide coatings on silica will be studied as a model of hard-tissue implants, and bioactive hydrogels formed by acrylamide polymers that have been modified with peptides will be studied to simulate tissue-scaffold materials. Peptides that contain the arginine-glycine-aspartate (RGD) are known to reduce inflammation, pain, immune response, and rejection when used as a biomaterial coating. A variety of RGD-coated silica and RGD-hydrogel samples will be characterized, first with the aim of understanding the rules of RGD presentation, with the ultimate goal of creating new silica-based and hydrogel biomaterials.Materials science and biological chemistry have converged with significant impacts, particularly with respect to advances in tissue implants and devices. Chemical analysis and NMR spectroscopy can enable new understanding of biomolecular structure, information that may lead to new biomaterial paradigms. This project includes an education plan that aims to expose high school, undergraduate, and graduate students to cutting edge research. This effort will include an outreach program to high school students and their teachers, involving the synthesis and evaluation of biomaterials. As well, the PI will develop new curricula at the undergraduate and graduate levels.

俄克拉荷马州大学的Charles Rice在实验物理化学计划的支持下，使用高分辨率魔角旋转和多维核磁共振（NMR）光谱技术来检查参与二维表面上细胞粘附和生长的特定肽的结构和动力学。 将研究二氧化硅上的肽涂层作为硬组织植入物的模型，并研究由用肽修饰的丙烯酰胺聚合物形成的生物活性水凝胶以模拟组织支架材料。已知含有精氨酸-甘氨酸-天冬氨酸（RGD）的肽在用作生物材料涂层时可以减少炎症、疼痛、免疫反应和排斥反应。 各种RGD涂层的二氧化硅和RGD-水凝胶样品将进行表征，首先是为了了解RGD呈现的规则，最终目标是创造新的基于二氧化硅和水凝胶的biomaterials.Materials science和biological chemistry have converged with significant impacts，especially with respect to advances in tissue implants and devices. 化学分析和NMR光谱学可以使生物分子结构的新的理解，可能导致新的生物材料范例的信息。 该项目包括一项教育计划，旨在让高中生、本科生和研究生接触前沿研究。 这项工作将包括针对高中生及其教师的外展计划，涉及生物材料的合成和评估。 此外，PI将在本科和研究生阶段制定新的课程。