SBIR Phase II: Kits for the Detection of Bioterror Pathogens

SBIR II 期：用于检测生物恐怖病原体的试剂盒

• 批准号: 0450469
• 负责人: Charles Spangler
• 金额: --
• 依托单位: SENSOPATH TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0450469&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Kits; Detection

This Small Business Innovation Research (SBIR) Phase II project proposes to develop field deployable kits for the detection of bio-terror pathogens. These kits would consist of fluorescent-labeled antibodies directed against protein toxins expressed by bio-terror pathogens, relying for detection on strong antibody-antigen interactions and fast chromatographic discrimination using simple chromatography strips supplied with inexpensive pre-measured reagents. In Phase I project, new water soluble blue-emitting reporter fluorophores were synthesized that were extremely photo-stable and could be easily visualized under any type of light conditions. These fluorophores were conjugated to an antibody against Bacillus anthracis as the initial proof-of-concept, and methodology was developed to attach these reporter fluorophores to monoclonal, polyclonal or recombinant antibodies. The objectives of Phase II project are to optimize reagents and chromatography, to synthesize new fluorophores for multiplexed pathogen detection, to design and assemble prototype kits, and to test and validate the kits.The commercial application of this project will be in the area of homeland security. The proposed kits are expected to be inexpensive, versatile, and easy to use by relatively untrained first responders (such as police, firefighters, paramedics, hazmat personnel, other emergency response teams).

这一小企业创新研究（SBIR）第二阶段项目提议开发用于检测生物恐怖病原体的现场部署套件。这些试剂盒将包括针对生物恐怖病原体表达的蛋白毒素的荧光标记抗体，依靠强抗体-抗原相互作用进行检测，并使用配有廉价预先测量试剂的简单色谱条进行快速色谱鉴别。在第一阶段项目中，合成了新的水溶性蓝光报告荧光团，它们具有极高的光稳定性，并且在任何类型的光条件下都可以轻松可视化。将这些荧光团缀合至针对炭疽芽孢杆菌的抗体作为初始概念验证，并开发了将这些报告荧光团连接至单克隆、多克隆或重组抗体的方法。第二阶段的目标是优化试剂和色谱，合成用于多重病原体检测的新荧光团，设计和组装原型试剂盒，并测试和验证试剂盒，该项目的商业应用将在国土安全领域。拟议的套件预计将是廉价的，通用的，易于使用的相对未经培训的第一响应者（如警察，消防员，护理人员，危险品人员，其他应急响应团队）。