SBIR Phase II: Detection and Identification Instrument for Single Molecule Analysis

SBIR第二期：单分子分析检测鉴定仪器

• 批准号: 0450539
• 负责人: Rajan Gurjar
• 金额: --
• 依托单位: Radiation Monitoring Devices Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0450539&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Detection; Identification

This Small Business Innovation Research (SBIR) Phase II project proposes to develop a novel, low cost laboratory instrument for genetic analysis and single molecule studies. The technology is suitable for the detection and identification of DNA and RNA through fluorescent hybridization probes without the need for Polymerase Chain Reaction (PCR) amplification, or for proteins and small molecules through fluorescence immunoassays. The general scheme is based on single molecule detection (SMD) and utilizes the two-color cross-correlation spectroscopy (TC-FCCS) technique with coincident detection analysis scheme to simultaneously probe ten focal regions of a microfluidic assay. High efficiency single photon detectivity Geiger mode microavalanche photodiode (uAPD) arrays will function as detection elements.The commercial application of this project will be on biological and medical research, and on the drug development process. Examples of potential applications range from the study of conformational dynamics and interactions of macromolecules to biochemical kinetics of single molecules.

这个小型企业创新研究(SBIR)第二阶段项目建议开发一种新的、低成本的实验室仪器，用于基因分析和单分子研究。该技术适用于通过荧光杂交探针检测和鉴定DNA和RNA，而不需要进行聚合酶链式反应(PCR)扩增，或者适用于通过荧光免疫分析检测蛋白质和小分子。一般方案是基于单分子检测(SMD)，并利用双色互相关光谱(TC-FCCS)技术和符合检测分析方案同时探测微流控分析的10个焦点区域。高效率的单光子探测盖革模微雪崩光电二极管阵列将作为探测元件，该项目的商业应用将在生物和医学研究以及药物开发过程中。潜在应用的例子从大分子的构象动力学和相互作用的研究到单分子的生化动力学。