Characterization of DNA-Linked Nanoparticle Networks for Advanced Genetic Testing

用于高级基因测试的 DNA 连接纳米颗粒网络的表征

• 批准号: 0503891
• 负责人: Joseph Wang
• 金额: $ 10.6万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-11-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0503891&HistoricalAwards=false
• 关键词: Characterization; DNA; Linked; Nanoparticle; Networks

Professor Joseph Wang of New Mexico State University is supported by the Analytical and Surface Chemistry Program to study nanoparticle-biopolymer assemblies for use as sensors. In particular, DNA hybridization events will be detected electrically on gold nanoparticles, with eventual applications in biodiagnostics and biodetection. New assays that couple multiple amplification pathways and an effective magnetic separation will allow for increased selectivity and sensitivity in the sensors. A new electrochemical coding technology will be developed for the simultaneous detection of multiple DNA targets. These projects will be combined on microfluidic device platforms. The ability to miniaturize complex chemical functions to achieve lab-on-a-chip is a goal of nanotechnology. In addition, this work can be related to biowarfare detection and thus has implications for national security.

新墨西哥州立大学的约瑟夫·王教授得到了分析和表面化学计划的支持，该计划研究用作传感器的纳米颗粒-生物聚合物组件。特别是，DNA杂交事件将在金纳米颗粒上进行电子检测，最终应用于生物诊断和生物检测。将多条放大路径和有效的磁分离相结合的新分析方法将允许提高传感器的选择性和灵敏度。将开发一种新的电化学编码技术，用于同时检测多个DNA靶标。这些项目将在微流控设备平台上合并。将复杂的化学功能微型化以实现芯片实验室的能力是纳米技术的目标。此外，这项工作可能与生物武器检测有关，因此对国家安全具有影响。