Characterization of Nanoparticle-Linked Biomolecule Assemblies for Advanced Bioelectronic Detection

用于先进生物电子检测的纳米颗粒连接生物分子组件的表征

• 批准号: 0840684
• 负责人: Joseph Wang
• 金额: $ 63.15万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0840684&HistoricalAwards=false
• 关键词: Characterization; Nanoparticle; Linked; Biomolecule; Assemblies

Professor Joseph Wang of Arizona State University is supported by the Analytical and Surface Chemistry program for the design of detectors of biomaterials with a focus on the detection of proteins. The group is applying different metal-sulfides for coding different proteins and analyzing simultaneously different antigens by the respective coded antibodies. This idea is being extended by combining magnetic beads/protein-protein complexes/metal labels as supramoleuclar structures that are magnetically collected on the electrode, and the collected metal conjugates are electrochemically analyzed. A further topic is the detection of single base polymorphism by the labeling of individual nucleotides with nanoparticle labels. Another topic includes the application of DNA barcodes for the amplified electrochemical detection of protein-protein interactions. The last topic in the program involves the construction of carbon nanotubes/enzymes layers on electrodes to increase the enzyme content on the electrodes, and electrically contact the proteins. Detection of biomaterials (proteins, peptides, nucleic acids) is of steadily growing interest, e.g. in the diagnostic of disease markers (tumor markers, viral antigens, therapy control) or the control of food safety, environmental pollutions or biohazards. Much effort is thus required to develop reliable, simple, fast and efficient tests to detect as low amounts as possible. Detecting trace amounts of proteins using straight-forward methodologies (e.g. electrochemically) is becoming increasingly important.

亚利桑那州立大学的Joseph Wang教授得到了分析和表面化学项目的支持，用于设计生物材料的检测器，重点是蛋白质的检测。该小组正在应用不同的金属硫化物编码不同的蛋白质，并通过各自编码的抗体同时分析不同的抗原。这一想法正在通过将磁珠/蛋白质-蛋白质复合物/金属标记物组合为超分子结构来扩展，所述超分子结构被磁性收集在电极上，并且所收集的金属缀合物被电化学分析。另一个主题是通过用纳米颗粒标记单个核苷酸来检测单碱基多态性。另一个主题包括DNA条形码在蛋白质-蛋白质相互作用的放大电化学检测中的应用。该计划的最后一个主题涉及在电极上构建碳纳米管/酶层，以增加电极上的酶含量，并与蛋白质电接触。生物材料（蛋白质、肽、核酸）的检测在例如疾病标志物（肿瘤标志物、病毒抗原、治疗控制）的诊断或食品安全、环境污染或生物危害的控制中具有稳定增长的兴趣。因此，需要作出很大努力来开发可靠、简单、快速和有效的检测方法，以检测尽可能低的含量。 使用直接方法（例如电化学）检测痕量蛋白质变得越来越重要。