Chromatography Opportunities for Learning and Understanding of Modern Separations (COLUMNS)

化学(12)在化学的几乎每一个分支学科和交叉学科领域，如制药学、法医学和环境科学，色谱学都是一种关键的方法。该项目为学生提供最常见的分离方法--高效液相色谱(HPLC)的接触和培训。一个垂直整合的色谱学实验室课程正在向化学和生物学生灌输对现代分离科学的深刻理解。这一协调的教育计划通过五门课程实施：普通化学、定量和仪器分析，以及两个生物化学实验室。这些课程跨越了化学和生物化学专业的课程。该项目正在改编基于文献的高效液相色谱实验，主要来自《化学教育杂志》，以创建一个连贯的课程，要求高级课程的学生应用他们在入门课程中学到的对色谱原理的理解。拟议课程的智力和教学价值在于，它通过两门入门课程：普通化学和定量分析，以及两门高级化学课程：仪器分析和生物化学，为学生提供高效液相色谱经验。鉴于该学院化学和生物化学课程的结构，学生因此有三门课程的色谱学经验。在这两门入门课程中，普通化学专业的学生探索分子极性并研究其如何直接影响现代仪器分析，而定量分析专业的学生则研究适用于高效液相色谱的色谱理论，并根据文献中的分析程序完成学生设计的期末专题。在高级化学课程中，生物化学专业的学生在第一学期实验室和学生设计的项目(第二学期实验室)中使用高效液相色谱法提纯和鉴定小肽；仪器分析专业的学生在辣椒素类化合物的分析和学生设计的项目中使用高效液相色谱法作为最终体验。在四门结合了新的高效液相色谱体验的课程中，有三门课程要么包含完全由学生推动的期末项目，要么完全以项目为导向。这个项目的广泛影响包括提供一个跨课程的教育计划，使学生能够学习分离方法的工作原理，并在其专业领域内的不同课程和子学科之间建立联系，在课程之间产生更紧密的联系；将现代分离方法和理论纳入化学课程；在以学生为中心、以学生为主导的项目中使用现代仪器；实施多课程评估计划，为该计划的教与学提供持续改进的反馈，并为参与评估的学生合作者提供新的和独特的学习经验；促进化学和生物教师之间的合作，以便为生物学生提供强大的色谱学经验；创造一个新的环境，让化学家和生物学家在教学和研究方面进行合作；并提供对不同的教职员工/学生研究项目有价值的新设施。

Chemistry (12) Chromatography is a key method in nearly every subdiscipline of chemistry and in interdisciplinary fields such as pharmaceuticals, forensics, and environmental science. This project is providing students with exposure to and training in the most common separation method, High Performance Liquid Chromatography (HPLC). A vertically integrated laboratory curriculum in chromatography is instilling a deep understanding of modern separation science in chemistry and biology students. This coordinated education program is being implemented in five courses: general chemistry, quantitative and instrumental analysis, and two biochemistry labs. These courses span the chemistry and biochemistry major curricula. The project is adapting literature-based experiments in HPLC, mostly from the Journal of Chemical Education, in order to create a coherent program that requires students in the advanced courses to apply their understanding of chromatographic principles learned in the introductory courses. The intellectual and pedagogic merit of the proposed curriculum is that it offers students HPLC experience in two introductory courses: general chemistry and quantitative analysis, and in two advanced chemistry courses: instrumental analysis and biochemistry. Given the structure of the chemistry and biochemistry curricula at the institution, students thus have chromatography experiences in three courses. In the two introductory courses, students in general chemistry explore molecular polarity and examine how it directly influences a modern instrumental analysis, and students in quantitative analysis examine the chromatographic theory applicable to HPLC and carry out student-designed final projects based on analytical procedures from the literature. In the advanced chemistry courses, students in biochemistry use HPLC to purify and characterize a small peptide (first semester lab) and in student-designed projects (second semester lab), and students in instrumental analysis use HPLC in the analysis of capsaicinoids and in student-designed projects that serve as a culminating experience. Of the four courses that are incorporating new HPLC experiences, three either include a final project wholly driven by the student or are completely project-oriented. The broader impacts of this project include providing a cross-curriculum educational program, which is enabling students to learn how separation methods work and also to make connections between different courses and subdisciplines within their major field, producing stronger ties between courses; incorporating modern separation methods and theory into the chemistry curriculum; using modern instruments in student-centered, student-driven projects; implementing a multi-course assessment plan that provides feedback for continued improvement of the teaching and learning in the program, and which is providing new and unique learning experiences for student collaborators involved in the evaluation; fostering collaborations between chemistry and biology faculty in order to provide biology students with strong experiences in chromatography; creating a new environment in which chemists and biologists are collaborating, in both teaching and research; and providing new facilities that are of value in diverse faculty/student research programs.