Molecular, Cellular, and Physiological studies of Multidrug-Resistance-like ABC Transporters in Arabidopsis Seedlings

拟南芥幼苗中类多药耐药性 ABC 转运蛋白的分子、细胞和生理学研究

• 批准号: 0517350
• 负责人: Edgar Spalding
• 金额: $ 39万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0517350&HistoricalAwards=false
• 关键词: Molecular; Cellular; Physiological; studies; Multidrug

The model plant Arabidopsis thaliana possesses 22 Multidrug Resistance (MDR)-like genes. Study of these genes is justified by the evidence, obtained with previous NSF support, that the MDR genes play an important role in the control of plant growth and development by controlling the flow and distribution of the plant growth hormone auxin, as well as by the possibility that the findings will shed light on basic cellular processes related to human health. (Over-expression of the animal MDR1 gene in cancer cells is linked to the development of drug-resistant tumors.) The activities proposed for the next funding cycle are focused on learning how the MDR genes (particularly MDR1 and MDR4) control the subcellular localization of the PIN auxin efflux regulators, which in turn control distribution of auxin. The work will result in a more detailed understanding of how auxin-dependent events at the cellular and molecular levels influence the form and shape of plants. The experiments will include 1) quantification of mdr mutant phenotypes using electronic imaging and custom computational software, 2) studies of protein localization and kinetics of trafficking using fluorescently-tagged MDR and PIN proteins, 3) structure-function studies of the cytoplasmic tail of MDR1 and MDR4 to learn about the targeting mechanism.In addition to the scientific goals of determining the functions of MDR proteins and how auxin controls plant growth and development, the project will include activities to promote a greater understanding of plants in the public at large and to stimulate youth to think about the science of plant biology. The personnel funded in this project have developed a traveling Discover Plants! outreach exhibit that can be set up at school science fairs. Through a mixture of media (live plants, microscopes, computer videos, and take-home projects), kindergarden through 8th grade students are exposed to various aspects of basic plant biology and its relevance to our everyday lives. Discover Plants! will be further developed and broadened to include more graduate student operators. Outreach at a higher scientific level will also occur via a website that will explain and demonstrate how measurements of shapes, plant shapes in particular, are made from a series of images. Downloadable software and some sample images will be provided. Links to and from engineering, mathematics, and computational sites will increase the exchange of information and ideas between these disciplines. Such a forum is expected to have a broad impact by attracting the attention of scientists in engineering and computational disciplines to issues in plant biology.

模式植物拟南芥（Arabidopsis thaliana）具有22个多药耐药（Multidrug Resistance，MDR）样基因。这些基因的研究是合理的证据，获得与以前的NSF的支持，MDR基因在控制植物生长和发育中发挥重要作用，通过控制植物生长激素生长素的流动和分布，以及通过的可能性，这些发现将揭示与人类健康有关的基本细胞过程。（动物MDR 1基因在癌细胞中的过度表达与耐药肿瘤的发展有关。下一个资助周期的活动重点是了解MDR基因（特别是MDR 1和MDR 4）如何控制PIN生长素流出调节剂的亚细胞定位，进而控制生长素的分布。这项工作将导致对细胞和分子水平上生长素依赖性事件如何影响植物的形态和形状的更详细的理解。实验将包括1）使用电子成像和定制计算软件定量mdr突变表型，2）使用荧光标记的MDR和PIN蛋白研究蛋白定位和运输动力学，3）结构-MDR 1和MDR 4细胞质尾区的功能研究，以了解靶向机制。除了确定MDR蛋白的功能和生长素如何控制植物的生长和发育，该项目将包括促进公众对植物的更多了解和激发青年对植物生物学科学的思考的活动。在这个项目中资助的人员已经开发了一个旅行发现植物！可以在学校科学展览会上设立的外展展览。通过媒体的混合物（活植物，显微镜，电脑视频，和带回家的项目），幼儿园通过8年级的学生接触到基本植物生物学的各个方面，它与我们的日常生活的相关性。发现植物！将进一步发展和扩大，以包括更多的研究生经营者。在更高的科学水平上的推广也将通过一个网站进行，该网站将解释和演示如何从一系列图像中测量形状，特别是植物形状。下载软件和一些样本图像将提供。工程、数学和计算网站之间的链接将增加这些学科之间的信息和思想交流。这样一个论坛预计将产生广泛的影响，吸引工程和计算学科的科学家对植物生物学问题的关注。