Collaborative Research: The Thermodynamics of Protein Separations

合作研究：蛋白质分离的热力学

• 批准号: 0518922
• 负责人: Michael Paulaitis
• 金额: $ 28.78万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2005-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0518922&HistoricalAwards=false
• 关键词: Collaborative; Research; Thermodynamics; Protein; Separations

Paulaitis0518922The objective of this research is to use measurements and mechanistic models of single and multicomponent protein solution thermodynamics as the basis for developing rational strategies for protein separation and purification. These strategies will build on the understanding of protein-protein interactions and of ways to manipulate and exploit them in designing effective separation pathways. This research will employ numerous approaches developed in earlier grant periods, including: methods to measure protein interactions, such as self-interaction chromatography; methods to calculate protein-protein interactions, including electrostatic, dispersion and hydration interactions; and methods to calculate phase diagrams from complex, anisotropic potentials such as those that apply to proteins. A particular area of emphasis in the research will be to relate the nature of protein interactions to the resulting phase diagram, as well as to the types of amorphous dense phases observed in practice, such as precipitates and gels.The project will support training of postdoctoral researchers and graduate students, as well as undergraduates. Such trainees in earlier grant periods have been successful in academic and industrial careers, and the undergraduates have been co-authors on numerous publications.

Paulaitis 0518922本研究的目的是使用单一和多组分蛋白质溶液热力学的测量和机理模型作为基础，为蛋白质分离和纯化制定合理的策略。这些策略将建立在对蛋白质-蛋白质相互作用的理解以及在设计有效分离途径时操纵和利用它们的方法的基础上。这项研究将采用早期资助期间开发的多种方法，包括：测量蛋白质相互作用的方法，如自相互作用色谱法;计算蛋白质-蛋白质相互作用的方法，包括静电，分散和水合作用;以及从复杂的各向异性势计算相图的方法，如适用于蛋白质的方法。该研究的一个特别重点领域是将蛋白质相互作用的性质与所得相图以及在实践中观察到的无定形致密相的类型（如沉淀物和凝胶）联系起来。该项目将支持博士后研究人员和研究生以及本科生的培训。这些学员在早期赠款期间在学术和工业生涯中取得了成功，本科生是许多出版物的共同作者。