Expedited Research for the Regeneration of Immunoadsorbents Using Elevated Pressures

利用高压加速免疫吸附剂再生的研究

• 批准号: 8918846
• 负责人: Michael Paulaitis
• 金额: $ 3.38万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-10-15 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8918846&HistoricalAwards=false
• 关键词: Expedited; Research; Regeneration; Immunoadsorbents; Using

Recent advances in molecular biology have led to the generation of new and novel techniques for producing biomedical and pharmaceutical proteins. Often, however, the requisite purity of such new materials dictates the need for a downstream separation technology well beyond the use of conventional or classical physicochemical recovery methods. In this work the focus is upon the effect of thermodynamic parameters such as pressure on the separation efficiency of these new biotechnology methods. The primary objective of this work is to investigate the effect of elevated pressures on the binding of protein agents (antigens, haptens, etc.) to biospecific receptors (monoclonal antibodies). Such studies will lead to the development of gentle methods of elution and recovery of these target biologicals.

分子生物学的最新进展导致了生产生物医学和药物蛋白质的新技术的产生。然而，通常情况下，这些新材料的纯度要求要求下游分离技术远远超出使用传统或经典的物理化学回收方法。在这项工作的重点是热力学参数的影响，如压力对这些新的生物技术方法的分离效率。这项工作的主要目的是研究压力升高对蛋白制剂（抗原、半抗原等）与生物特异性受体（单克隆抗体）结合的影响。这样的研究将导致温和的方法洗脱和回收这些目标生物制品的发展。