ME: Engineering E. coli to Maximize the Flux of Reducing Equivalents Available for NAD(P)H-Dependent Transformations

ME：工程大肠杆菌以最大化可用于 NAD(P)H 依赖性转化的还原当量通量

• 批准号: 0519516
• 负责人: Patrick Cirino
• 金额: $ 63.57万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-15 至 2009-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0519516&HistoricalAwards=false
• 关键词: ME; Engineering; E.; coli; Maximize

Cirino0519516This research is to resolve issues regarding enzymatic transformations catalyzed by reduced nicotinamide cofactor dependent reductases, dehydrogenases, and oxygenases. It is proposed to metabolically engineer E. coli strains such that sugars will be completely oxidized without fermentation end products and the resulting reducing equivalents diverted away from aerobic respiration toward driving other reactions of interest. A broader impact of the research is the development of viable routes to renewable fuels and chemicals by optimizing the efficiency with which biomass is utilized for medically and industrially significant biotransformations. Computational modeling will guide design efforts and experimental results will serve to correct and complete the modeling frameworks.

Cirino0519516本研究旨在解决由还原的烟酰胺辅因子依赖还原酶、脱氢酶和加氧酶催化的酶转化问题。有人建议对大肠杆菌菌株进行新陈代谢改造，使糖在没有发酵最终产物的情况下完全氧化，由此产生的还原当量从有氧呼吸转向驱动其他感兴趣的反应。这项研究的一个更广泛的影响是，通过优化生物质用于医学和工业上重大生物转化的效率，开发了获得可再生燃料和化学品的可行途径。计算建模将指导设计工作，实验结果将用于纠正和完善建模框架。