Selective Filling of Nanostructured Packings for Chromatographic Chip Systems

建议标题：用于色谱芯片系统的纳米结构填料的选择性填充建议编号：CTS-0522656主要研究人员：Steven CramerInstitution：Rensselaer理工学院该项目将采用微制造、选择性填充和溶胶-凝胶纳米技术来开发各种用于生物分离应用的高效色谱芯片系统。初步结果表明，一种新的选择性填充技术可以用来在色谱芯片系统中产生复杂的图案。用于制造具有独特性能的溶胶-凝胶粘结剂的最先进技术将被开发用于色谱芯片系统。高效的色谱芯片系统将通过使用溶胶-凝胶技术固定小颗粒直径的色谱材料以及增加色谱柱长度和梯度操作来开发。将使用生物测试混合物评估色层芯片系统的孔形态、渗透性、孔径分布、色层效率和分离能力。多功能系统将使用选择性填充溶胶凝胶技术进行检测，以芯片形式将酶消化与梯度层析相结合。就更广泛的影响而言，该项目可能会指导开发一种新的平台技术，以实现多功能芯片格式的色谱分离，这将对从蛋白质组学到环境科学的各种领域产生潜在影响。此外，选择性填充溶胶-凝胶固定化技术的发展将促进芯片实验室器件技术的进步。该项目还将教育本科生和研究生了解最先进的溶胶-凝胶纳米技术以及基于芯片的分离系统。色谱芯片技术可以影响生物分离很重要的领域，包括环境科学和蛋白质组学。

AbstractProposal Title: Selective Filling of Nanostructured Packings for Chromatographic Chip SystemsProposal Number: CTS-0522656Principal Investigator: Steven CramerInstitution: Rensselaer Polytechnic InstituteThis project will employ microfabrication, selective filling and sol-gel nanotechnology to develop a variety of highly efficient chromatographic chip systems for bioseparations applications. Preliminary results indicate that a novel selective filling technique can be employed to create complex patterns in chromatographic chip systems. State of the art techniques employed for making sol-gel binders with unique properties will be developed for chromatographic chip systems. Efficient chromatographic chip systems will be developed by the immobilization of small particle diameter chromatographic materials using sol-gel techniques along with increased column lengths and gradient operation. The chromatographic chip systems will be evaluated for pore morphology, permeability, pore size distribution, chromatographic efficiency, and separation capability using biological test mixtures. Multifunctional systems will be examined using the selective filling sol-gel technique for integrating enzymatic digestion with gradient chromatography in a chip format. In terms of the broader impacts, this project may guide the development of a new platform technology for implementing chromatographic separations in multifunctional chip formats, which will have a potential impact on a variety of fields ranging from proteomics to environmental science. In addition, the development of selective filling sol-gel immobilization technology will facilitate advances in the state of the art of lab on chip devices. The project will also educate both undergraduate and graduate students in the state of the art of sol-gel nanotechnology as well as chip based separation systems. Chromatographic chip technology can impact areas where bioseparations are important, including environmental science and proteomics.