CRC: Characterization of Changes in Molecular Structure and Activity of Autoinducer Molecules Relevant to Bacterial Chemical Communication

CRC：与细菌化学通讯相关的自诱导分子的分子结构和活性变化的表征

• 批准号: 0526821
• 负责人: Alan Decho
• 金额: $ 184万
• 依托单位: University South Carolina Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0526821&HistoricalAwards=false
• 关键词: CRC; Characterization; Changes; Molecular; Structure

This award supports the collaborative efforts of Alan Decho, Stanley Angel, Lee Ferguson and John Ferry, all at the University of South Carolina, to understand autoinducer molecules, how these molecules are transformed under environmental conditions, and how the molecules interact with protein receptors. Acylated homoserine lactones, AHLs, are a family of molecules present in gram-negative bacteria. The AHLs allow one bacterium to communicate with others nearby, leading to quorum sensing. This project will address four major questions: 1) How do specific environmental stressors such as light, pH, humidity and metal ions modify AHL molecules; (2) How do changes in the AHLs alter complexation to regulatory proteins; (3) Which changes in AHL/Protein Binding result in changes in gene expression; and (4) Can Raman and fluorescence-lifetime signatures be used to image and quantify in situ intact and altered AHLs?This project is funded through the Collaborative Research in Chemistry Program (CRC) and provides outstanding opportunities for undergraduates and graduate students to acquire knowledge and skills in very broad areas of environmental analytical chemistry, spectroscopy and microbiology. New techniques, including high-throughput multivariate environmental transformation studies and new spectroscopic tools that can detect low levels of AHLs in complex environmental samples will be developed. The implications of this chemical signalling pathway in microbial mats and other environmental settings will be explored.

该奖项支持南卡罗来纳州大学的Alan Decho，Stanley Angel，Lee Ferguson和John Ferry的合作努力，以了解自诱导分子，这些分子如何在环境条件下转化，以及分子如何与蛋白质受体相互作用。酰化高丝氨酸内酯（AHLs）是存在于革兰氏阴性细菌中的分子家族。AHLs允许一个细菌与附近的其他细菌交流，从而导致群体感应。本项目将解决四个主要问题：1）特定的环境应激因素如光、pH、湿度和金属离子如何改变阿勒分子; 2）AHL的变化如何改变与调节蛋白的复合; 3）AHL/蛋白结合的哪些变化导致基因表达的变化; 4）AHL的变化如何影响基因表达;和（4）拉曼和荧光寿命特征是否可用于原位成像和定量完整和改变的AHL？该项目通过化学合作研究计划（CRC）资助，为本科生和研究生提供了在环境分析化学，光谱学和微生物学等广泛领域获得知识和技能的绝佳机会。将开发新技术，包括高通量多变量环境转化研究和新的光谱工具，以检测复杂环境样品中的低水平AHLs。将探讨这种化学信号通路在微生物垫和其他环境设置的影响。