Analysis of canine peripheral CD4+CD8+ double-positive T cell phenotype and function

犬外周血CD4 CD8双阳性T细胞表型及功能分析

• 批准号: 173176068
• 负责人: Professor Dr. Gottfried Alber
• 金额: --
• 依托单位: Institut für Immunologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/173176068?language=en
• 关键词: Analysis; canine; peripheral; CD4+CD8+; double

T lymphocytes co-expressing CD4 and CD8 (termed double-positive T cells, dp T cells) are commonly associated with a thymic developmental stage of T cells. The first description of dp T cells as extrathymic T cells almost 30 years ago came as a surprise. Peripheral dp T cells have been characterized best in humans and swine. In our very recent studies on canine peripheral dp T cells we unraveled unique features of dp T cells present in dogs but not in swine or humans. Canine CD4+CD8+ T cells can be divided into three different cellular subsets with distinct functional profiles (e.g., production of interferon-gamma), distinct progenitors (CD4+ as well as CD8+ single-positive T cells), distinct expression of markers indicative of effector vs. memory status, or of cytotoxic or regulatory function. The objectives of this proposal are to (i) complete the functional analysis, (ii) complete the characterization of the tissue distribution (lymphatic tissue vs. non-lymphatic tissue) with an emphasis on intestinal mucosal dp T cells, (iii) define subset-specific transcription factors responsible for regulation of CD4 or CD8, and (iv) analyze the in vivo role of dp T cells in a frequent chronic canine inflammatory disease such as idiopathic inflammatory bowel disease.

共同表达CD4和CD8的T淋巴细胞（称为双阳性T细胞，dp T细胞）通常与T细胞的胸腺发育阶段有关。大约30年前，第一次将dp T细胞描述为胸腺外T细胞是令人惊讶的。外周dp T细胞在人和猪中表现最好。在我们最近对犬外周dp T细胞的研究中，我们揭示了dp T细胞存在于犬但不存在于猪或人类中的独特特征。犬CD4+CD8+ T细胞可分为三个不同的细胞亚群，具有不同的功能特征（例如，产生干扰素γ），不同的祖细胞（CD4+和CD8+单阳性T细胞），不同的标记物表达，表明效应与记忆状态，或细胞毒性或调节功能。本提案的目标是(i)完成功能分析，（ii）完成组织分布（淋巴组织与非淋巴组织）的表征，重点是肠粘膜dp T细胞，（iii）定义负责调节CD4或CD8的亚群特异性转录因子，（iv）分析dp T细胞在犬类常见慢性炎症性疾病（如特发性炎症性肠病）中的体内作用。

项目成果

Canine peripheral blood CD4+CD8+ double-positive Tcell subpopulations exhibit distinct Tcell phenotypes and effector functions.

犬外周血CD4 CD8双阳性T细胞亚群表现出不同的T细胞表型和效应功能

• DOI: 10.1016/j.vetimm.2017.01.005
• 发表时间: 2017
• 期刊: Veterinary immunology and immunopathology
• 影响因子: 1.8
• 作者: Rothe K;Bismarck D;Büttner M;Alber G;von Buttlar H
• 通讯作者: von Buttlar H

Distinct Features of Canine Non-conventional CD4−CD8α− Double-Negative TCRαβ+ vs. TCRγδ+ T Cells

犬非常规 CD4âCD8α 双阴性 TCRαβ 与 TCRγδ T 细胞的独特特征

• DOI: 10.3389/fimmu.2019.02748
• 发表时间: 2019
• 期刊: Frontiers in Immunology
• 影响因子: 7.3
• 作者: Rabiger FV;Rothe K;von Buttlar H;Bismarck D;Büttner M;Moore PF;Eschke M;Alber G
• 通讯作者: Alber G

Canine tissue-associated CD4+CD8α+ double-positive T cells are an activated T cell subpopulation with heterogeneous functional potential

• DOI: 10.1371/journal.pone.0213597
• 发表时间: 2019-03-13
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Rabiger, Friederike V.;Bismarck, Doris;Eschke, Maria
• 通讯作者: Eschke, Maria