Secretion Signals and Type III Chaperones in the Pseudomonas Syringae Type III Secretion System

丁香假单胞菌 III 型分泌系统中的分泌信号和 III 型伴侣

• 批准号: 0544447
• 负责人: James Alfano
• 金额: $ 42万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0544447&HistoricalAwards=false
• 关键词: Secretion; Signals; Type; III; Chaperones

Type III protein secretion systems (TTSSs) of Gram-negative plant and animal pathogens inject bacterial proteins, called effectors, into eukaryotic cells resulting in the modulation of host signal transduction pathways. Effector proteins are targeted to the TTSS by secretion signals carried in their N-termini and/or with the aid of specialized accessory proteins called type III chaperones (TTCs). Increasing our understanding of these processes may ultimately help in the design of pharmaceuticals and/or agricultural pesticides or herbicides. Thus, the intellectual merit of the proposed activity is quite significant because increasing our understanding of this topic may lead to better control of plant and animal diseases caused by pathogens dependent on TTSS. Project experiments were designed to explore the secretion signals used by effectors and the involvement of TTCs in the TTSS of the bacterial plant pathogen Pseudomonas syringae. The goal of several experiments is to determine the biological function of type III secretion signals. These experiments will utilize several different substrates of TTSSs, and will be focused on the role that N-terminal secretion signals and/or TTCs (and their binding sites) play in the secretion and translocation (injection into host cells) of effectors. The experiments in this project offer comprehensive training for students. The PI has initiated collaborations with a Nebraska program that targets the involvement of underrepresented minority students as well as collaborations with professors at local liberal arts colleges to reach students that may not have ample opportunities for a research experience.

革兰氏阴性植物和动物病原体的III型蛋白分泌系统（TTSS）将称为效应子的细菌蛋白质注射到真核细胞中，导致宿主信号转导途径的调节。效应蛋白通过其N-末端携带的分泌信号和/或在称为III型分子伴侣（TTC）的专门辅助蛋白的帮助下靶向TTSS。增加我们对这些过程的理解可能最终有助于药物和/或农业杀虫剂或除草剂的设计。因此，拟议活动的知识价值是相当重要的，因为增加我们对这一专题的了解可能导致更好地控制依赖TTSS的病原体引起的动植物疾病。设计了项目实验，以探索细菌性植物病原菌假单胞菌的TTSS中效应子使用的分泌信号和TTCs的参与。几个实验的目的是确定III型分泌信号的生物学功能。这些实验将利用TTSS的几种不同底物，并将集中于N-末端分泌信号和/或TTC（及其结合位点）在效应物的分泌和易位（注射到宿主细胞中）中所起的作用。该项目的实验为学生提供了全面的训练。PI已经开始与内布拉斯加州的一个项目合作，该项目的目标是代表性不足的少数民族学生的参与，以及与当地文理学院教授的合作，以接触那些可能没有足够机会获得研究经验的学生。