CAREER: Engineering and Integrated Microfluidic System for High Throughput Genomic Analyses

职业：用于高通量基因组分析的工程和集成微流体系统

• 批准号: 0547193
• 负责人: Xiaohua Huang
• 金额: $ 40万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0547193&HistoricalAwards=false
• 关键词: CAREER; Engineering; Integrated; Microfluidic; System

0547193HuangThe overall goal of this research is to develop a multidisciplinary program to create such a new paradigm with two specific objectives: (1) To engineer microfluidic devices for whole genome-scale DNA amplification, and (2) To develop an integrated system for high throughput genome sequencing and digital gene expression profiling. Microfabrication tools will be used to engineer high density arrays of femtoliter wells on polydimethylsiloxane (PDMS) substrates for whole genome-scale DNA amplification. Single DNA molecules from a genomic DNA or cDNA library will be cloned in massive parallel on the microfluidic devices using the rolling circle amplification (RCA) technology developed by the investigator. A new sequencing technology called "sequencing by denaturation" (SBD) will be developed to enable the massive parallel sequencing of the single molecular clones on the whole genome or transcriptome array. These technologies will be integrated with a high throughput imaging system to engineer an integrated system for genomic analyses.

这项研究的总体目标是开发一个多学科计划，以创建这样一个新的范例，有两个具体的目标：(1)设计用于全基因组规模DNA扩增的微流控设备，(2)开发一个用于高通量基因组测序和数字基因表达谱的集成系统。微制造工具将被用于在聚二甲基硅氧烷(PDMS)底物上设计高密度的飞升井阵列，用于全基因组规模的DNA扩增。利用研究人员开发的滚环扩增(RCA)技术，将在微流控设备上大规模并行克隆基因组DNA或cDNA文库中的单个DNA分子。一种名为“变性测序”(SBD)的新测序技术将被开发出来，以实现对整个基因组或转录组阵列上的单分子克隆的大规模并行测序。这些技术将与高通量成像系统集成，以设计一个用于基因组分析的集成系统。