CAREER: Production of Therapeutic T Cells from Mouse Embryonic Stem Cells: A Bioengineering Approach

职业：利用小鼠胚胎干细胞生产治疗性 T 细胞：一种生物工程方法

• 批准号: 0547252
• 负责人: Krishnendu Roy
• 金额: $ 40万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0547252&HistoricalAwards=false
• 关键词: CAREER; Production; Therapeutic; Cells; Mouse

Roy0547252The objective of this CAREER award is to develop efficient bioengineering tools to direct mouse embryonic stem (ES) cells to hematopoietic progenitor cells (HPCs) and ultimately to therapeutic, antigen-specific T cells suitable for immunotherapy and transplantation. In particular, the effects of 3D scaffold structures and various bioreactor cultures on hematopoietic differentiation of ES cells will be studied. Artificial thymus-like environments will be created using microbead-based signaling (synthetic thymic stromal cell) and tetramer-directed antigen presentation (artificial antigen presenting cell) to direct ES cell-derived HPCs into functional T cells. The research objectives, if successful, would lead to the development of scalable methods for generation of hematopoietic stem cells and T cells from ES cells. This could ultimately provide a readily available source for on demandadministration of therapeutic cells in a variety of complex clinical disorders, especially cancers. The educational objectives include community outreach programs for 9th and 10th graders, involving graduate and undergraduate biomedical engineering students and learning scientists from the University of Texas as well as science teachers from area high schools. The PI will also develop challenge-centered teaching modules based on how people learn (HPL) concepts through regular workshops involving high school science teachers and their students.

Roy0547252该职业奖的目标是开发有效的生物工程工具，将小鼠胚胎干细胞（ES）定向为造血祖细胞（HPC），并最终定向为适用于免疫治疗和移植的治疗性抗原特异性T细胞。特别地，将研究3D支架结构和各种生物反应器培养物对ES细胞造血分化的影响。将使用基于微珠的信号传导（合成胸腺基质细胞）和四聚体定向抗原呈递（人工抗原呈递细胞）来产生人工胸腺样环境，以将ES细胞衍生的HPC引导成功能性T细胞。研究目标，如果成功，将导致开发可扩展的方法，用于从ES细胞产生造血干细胞和T细胞。这最终可以为各种复杂的临床疾病，特别是癌症的治疗细胞的按需给药提供一个容易获得的来源。教育目标包括为9年级和10年级学生提供的社区外展计划，涉及研究生和本科生生物医学工程专业的学生和德克萨斯大学的学习科学家以及地区高中的科学教师。PI还将通过涉及高中科学教师及其学生的定期研讨会，根据人们如何学习（HPL）概念开发以挑战为中心的教学模块。