SBIR Phase II: Enabling High Output Metabolism in Plant Cells

这个小企业创新研究（SBIR）二期项目旨在开发和验证一种新的叶绿体转化载体，用于在叶绿体中表达蛋白质。该研究项目将通过解决多种转基因的化学计量表达，为胡萝卜素生成、反馈调节和多聚体蛋白表达等途径的有效工程，拓宽对叶绿体转化参数的科学理解。这项技术的商业影响将为叶绿体中感兴趣的基因的表达提供一种可行的策略，从而潜在地增加高价值营养和药物化合物的生产。该技术的稳定、高产量代谢和合规应用将降低生产成本，提高下游加工和最终商业化的可靠性。

This Small Business Innovation Research (SBIR) Phase II project aims to develop and validate a novel chloroplast transformation vector for protein expression in chloroplasts. The research project will broaden scientific understanding of the parameters of chloroplast transformation by addressing stoichiometric expression of multiple transgenes for effective engineering of pathways such as carotenogenesis, feedback regulation and expression of multimeric proteins.The commercial impact of this technology will provide an enabling strategy for expression of genes of interest in chloroplasts to potentially increase the production of high value nutraceutical and pharmaceutical compounds. Application of this technology for stable, high output metabolism with regulatory compliance will reduce production cost and increase the reliability for downstream processing and eventual commercialization.