Single-molecule fluorescence spectroscopy and imaging of the structure and dynamics of macromolecular assemblies (A06)

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The core goal of the project is the development of a fluorescence-optical method for three-dimensional localization and co-localization of single fluorescent molecules with ~1 nm isotropic resolution. For this purpose, we will combine two techniques: single-molecule localization based super-resolution microscopy (STORM, PALM, PAINT, MinFlux) with metal Induced Energy Transfer (MIET) imaging. We will use this method to image structural details of fluorescently labelled macromolecular complexes with nanometer resolution and high specificity, but also to resolve temporal dynamics of structural changes with a temporal resolution down to a few nanoseconds.