Function and properties of the adhesion-G-protein-coupled receptor latrophilin in the fruit fly Drosophila melanogaster

果蝇粘附 G 蛋白偶联受体 Latrophilin 的功能和特性

• 批准号: 174980912
• 负责人: Professor Dr. Tobias Langenhan
• 金额: --
• 依托单位: Rudolf-Schönheimer-Institut für Biochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/174980912?language=en
• 关键词: Function; properties; adhesion; protein; coupled

This project aims to investigate the molecular function of latrophilins, an individual class of adhesion-GPCRs, which are involved in synaptic neurotransmission and tissue polarization during development. To date it is unclear whether latrophilins exert their role in neural tissue by controlling synaptic exocytotic events, by participating in developmental functions, or by alternative means. Recent results on latrophilin function obtained in the roundworm C. elegans have uncovered essential developmental roles for latrophilins during neuroectodermal development and show that latrophilins partake in the establishment of primordial polarity. A possible role for latrophilins in mechanisms that govern the establishment or maintenance of structural and functional synaptic polarity has not been addressed to date. In the course of the proposed project the hypothesis will be tested whether the latrophilin receptor homolog of the fruitfly Drosophila melanogaster engages in the establishment tissue polarity ofthe eye and epidermal appendages ofthe abdomen and the wing. Secondly, the impact of latrophilin removal at a defined and well-characterized synaptic layout, the neuromuscular junction of Drosophila, will be investigated. This set of experiments will address the hypothesis that latrophilin function is involved in synaptic vesicle exocytosis. The project objectives require the generation of transgenic fly strains, structural and ultrastructural microscopy, in vivo imaging, and high-resolving electrophysiology.

该项目旨在研究latrophilins的分子功能，latrophilins是一类单独的粘附GPCR，参与发育过程中的突触神经传递和组织极化。到目前为止，它是不清楚是否latrophilins发挥其作用，在神经组织中通过控制突触胞吐事件，通过参与发育功能，或通过替代手段。蛔虫中latrophilin功能的最新研究结果。elegans已经揭示了亲latrophilins在神经外胚层发育过程中的重要发育作用，并表明亲latrophilins参与了原始极性的建立。latrophilins的可能作用机制，管理建立或维持结构和功能突触极性尚未解决的日期。在拟议的项目的过程中，假设将被测试是否亲latrophilin受体同源物的果蝇黑腹果蝇参与建立组织极性的眼睛和表皮附属物的腹部和翅膀。其次，将研究在果蝇的神经肌肉接头处的定义和良好表征的突触布局中去除latrophilin的影响。这组实验将解决这一假设，即latrophilin功能参与突触囊泡胞吐。该项目的目标需要产生转基因苍蝇品系，结构和超微结构显微镜，在体内成像，和高分辨率的电生理学。