A CONTINUOUS FLOW MICROREACTOR FOR LINEAR RNA AMPLIFICATION

用于线性 RNA 扩增的连续流微反应器

• 批准号: 0555874
• 负责人: Anubhav Tripathi
• 金额: $ 29.97万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0555874&HistoricalAwards=false
• 关键词: CONTINUOUS; FLOW; MICROREACTOR; LINEAR; RNA

0555874TripathiThe goal of this research is to develop a continuous flow microchip reactor for producing RNA transcripts of cDNA. The idea is to linearly amplify RNA through its conversion to cDNA and then linearly amplify this by repeated transcription of new RNA. This has advantages over PCR, in that the amplification is linear in time or throughput rather than exponential as in PCR, and thus less prone to bias than is PCR. Preliminary results include creation of a microfluidic particle-bed reactor, and mastery of the basic biochemical reactions. The proposed work includes considerable optimization of channel dimensions and reaction conditions. One aim of the research is the creation of a kinetic model of the reactor.

0555874 Tripath本研究的目的是开发一种用于生产cDNA的RNA转录物的连续流动微芯片反应器。 这个想法是通过将RNA转化为cDNA来线性扩增RNA，然后通过重复转录新RNA来线性扩增。 这具有优于PCR的优点，因为扩增在时间或通量上是线性的，而不是像PCR中那样是指数的，因此比PCR更不容易产生偏差。 初步结果包括创建一个微流控颗粒床反应器，并掌握基本的生化反应。 建议的工作包括相当大的优化通道尺寸和反应条件。 研究的一个目的是建立反应器的动力学模型。