The Maize TILLING Project: Reverse genetics of maize point mutations

单碱基变化是功能基因组学中有价值的工具，因为它们可以揭示基因产物活性、相互作用和特异性的变化。靶向诱导有限病变基因组（TILLING）是一种技术，可以筛选大量的突变生物体，以寻找那些在任何感兴趣的基因中具有单个碱基变化的个体。玉米耕作项目（MTP）提供了一项服务，使玉米研究人员能够识别和获得具有任何基因缺陷的突变品种的种子。该奖项旨在使MTP在经济上自给自足。此外，MTP正在创建和评估B73和W22自交系的新诱变系，为社会提供最佳诱变种子的一般资源。这些突变系将通过强调服务不足的社区和中学学生和教师的外展计划进行详细表型分析。获取项目成果：所有表型和突变数据将公开提供（http://genome.purdue.edu/maizetilling）。TILLING客户收到订单六个月后，序列数据将发布在Fred Hutchinson癌症研究中心（http://fhcrc.org:9355/search.html）和MaizeGDB （http://www.plantgdb.org/ZmGDB/）的TILLING主服务器上。库存将通过玉米遗传合作库存中心（http://w3.ag.uiuc.edu/maize-coop/）提供。

PI: Cliff Weil, Purdue University Single base changes are valuable tools in functional genomics because they can reveal changes in gene product activity, interaction and specificity. Targeting Induced Limited Lesions IN Genomes (TILLING) is a technology that can screen large populations of mutagenized organisms for those individuals that have single base changes in any gene of interest. The Maize TILLING Project (MTP) provides a service allowing maize researchers to identify and obtain seed for mutant varieties with defects in any gene. This award is designed to allow the MTP to become financially self-supporting. In addition, MTP is creating and evaluating new mutagenized lines of the inbreds B73 and W22 to provide the community with a general resource of optimally mutagenized seed. These mutant lines will be phenotyped in detail through an outreach program emphasizing underserved communities and secondary school students and teachers. Access to project outcomes: All phenotypic and mutation data will be made publicly available (http://genome.purdue.edu/maizetilling). Six months after the TILLING client has received his/her order, sequence data will be posted at the main TILLING server at Fred Hutchinson Cancer Research Center (http://fhcrc.org:9355/search.html) and MaizeGDB (http://www.plantgdb.org/ZmGDB/). Stocks will be made available through the Maize Genetics Cooperation Stock Center (http://w3.ag.uiuc.edu/maize-coop/).