Characterization of the Tomato Secretome Using Integrated Functional and Computational Strategies

使用集成功能和计算策略表征番茄分泌组

• 批准号: 0606595
• 负责人: Jocelyn Rose
• 金额: $ 362.7万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0606595&HistoricalAwards=false
• 关键词: Characterization; Tomato; Secretome; Using; Integrated

The plant cell wall plays a central role in many diverse aspects of plant biology, including organ growth and differentiation, defence against microbial pathogens, metabolism and transport of nutrients and responses to environmental stresses. In addition, the plant cell wall can be thought of as a vital compartment that acts as the interface with the environment and so substantial molecular signaling occurs in and across the wall environment. In accordance with its multifunctional nature, the plant cell wall houses a large and complex protein population, or proteome, including enzymes with a broad range of catalytic functions as well as structural proteins. However, it is increasingly evident that there is still a very limited understanding of the extent, composition and dynamic properties of the cell wall proteome, also termed the 'secretome'. This project will use an integrated approach to catalog the tomato secretome, coupling a suite of newly developed functional screens with sequencing of highly purified wall protein extracts, bioinfomatic tools and computational prediction. This infrastructure will be used to evaluate the qualitative and quantitative dynamics of the cell wall proteome during two biological processes that are intimately associated with wall biology: fruit ripening and plant defence responses. To this end, protein and mRNA expression will be studied in a standard experimental tomato genotype and contrasted with those in several non-softening tomato mutants.This research will generate a comprehensive catalog of the plant secretome in a range of tomato tissues and importantly, provide insights into the extracellular proteins that appear to contribute to, and play a role in, signaling during fruit ripening and in response to pathogen attack. The resulting information should allow important insights into how to improve fruit quality and agricultural productivity. A publicly accessible tomato secretome database (SecreTom) will be developed to house the project data, to provide access to all associated computational tools, and to act as a hub for plant wall proteome research. SecreTom will be accessible through the Solanaceous Genomes Database (SGN: http://www.sgn.cornell.edu/), which will also serve as the long-term repository for the project data. In addition, an annual summer Proteomics Workshop will be held, comprising a seminar series, poster presentations, and lab-based practical training. The participation of under-represented groups at the undergraduate and graduate levels will be ensured through the support of a network of existing Cornell programs.

植物细胞壁在植物生物学的许多不同方面起着核心作用，包括器官生长和分化、对微生物病原体的防御、营养物质的代谢和运输以及对环境胁迫的反应。此外，植物细胞壁可以被认为是一个重要的隔室，作为与环境的界面，因此大量的分子信号发生在壁环境中和穿过壁环境。根据其多功能的性质，植物细胞壁容纳了大量复杂的蛋白质群体或蛋白质组，包括具有广泛催化功能的酶以及结构蛋白。然而，越来越明显的是，仍然有一个非常有限的了解的范围，组成和动态特性的细胞壁蛋白质组，也被称为'分泌组'。该项目将使用一种综合方法对番茄分泌组进行编目，将一套新开发的功能筛选与高度纯化的壁蛋白提取物的测序、生物信息学工具和计算预测相结合。该基础设施将被用来评估定性和定量动态的细胞壁蛋白质组在两个生物过程中，是密切相关的壁生物学：果实成熟和植物防御反应。为此，蛋白质和mRNA的表达将研究在一个标准的实验番茄基因型和对比，在几个非软化番茄突变体。这项研究将产生一个全面的目录的植物分泌组在一系列番茄组织和重要的是，提供见解的胞外蛋白质，似乎有助于，并发挥作用，信号在果实成熟和响应病原体的攻击。由此产生的信息应有助于深入了解如何提高水果质量和农业生产力。将开发一个可公开访问的番茄分泌组数据库（SecreTom）来存储项目数据，提供所有相关计算工具的访问权限，并作为植物壁蛋白质组研究的中心。 SecreTom将通过茄科基因组数据库（SGN：http：//www.sgn.cornell.edu/）访问，该数据库也将作为项目数据的长期储存库。此外，还将举办一年一度的夏季蛋白质组学研讨会，包括系列研讨会，海报展示和基于实验室的实践培训。代表性不足的群体在本科生和研究生水平的参与将通过现有康奈尔计划网络的支持得到确保。