CBA: QEIB: Estimation and Interpretation of Allele Frequencies: Novel Theoretical Analyses and High-Throughput Quantitation by DNA Pooling

CBA：QEIB：等位基因频率的估计和解释：新颖的理论分析和通过 DNA 池进行高通量定量

• 批准号: 0609722
• 负责人: John Kelly
• 金额: --
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0609722&HistoricalAwards=false
• 关键词: CBA; QEIB; Estimation; Interpretation; Allele

Simple sequence repeats (SSRs) are repetitive sequences of DNA that have been found throughout the genome. SSRs are studied in diverse scientific settings in many kinds of organisms. Relative to the standard approach of individual genotyping, protocols using SSR DNA pooled from multiple individuals have the potential to improve laboratory efficiency (in terms of time and money) for many kinds of population genetic studies. This study investigates laboratory methods for estimating SSR allele frequencies using pooled striped bass DNA to determine optimal protocols. A complementary set of mathematical analyses will investigate the estimation of associations among alleles, the assessment of genetic structure among subpopulations, alternative stochastic models for genetic drift, and the spatial rate of spread of novel, advantageous genes into structured populations.Allele frequency estimation by DNA pooling has the potential to facilitate a wide variety of genetic investigations, including applications in disease mapping, forensic science, virology, and microbial ecology. Moreover, striped bass is economically important in the fishing and aquaculture industries, and the mathematical analyses will facilitate population genetic studies concerning the management of this and other species whose populations are of conservation and/or economic importance. Finally, this research is interdisciplinary, involving investigators with experience in statistics and genetics, physiology and biotechnology, and population ecology and genetics, and the expectation is that the senior personnel and their students, as well as their science, will benefit from the interaction.

简单重复序列（SSR）是在整个基因组中发现的DNA重复序列。SSR在多种生物中的不同科学环境中进行了研究。相对于个体基因分型的标准方法，使用从多个个体汇集的SSR DNA的协议有可能提高实验室效率（在时间和金钱方面），用于许多种群体遗传学研究。本研究探讨了实验室方法估计SSR等位基因频率使用池条纹低音DNA，以确定最佳的协议。一组互补的数学分析将研究等位基因之间关联的估计，亚群之间遗传结构的评估，遗传漂变的替代随机模型，以及新的、有利的基因进入结构化群体的空间传播率。通过DNA池化进行等位基因频率估计有可能促进各种各样的遗传研究，包括疾病作图，法医学、病毒学和微生物生态学。此外，条纹鲈鱼在渔业和水产养殖业中具有重要的经济意义，数学分析将有助于种群遗传学研究，这些研究涉及该种群和其他种群具有保护和/或经济重要性的物种的管理。最后，这项研究是跨学科的，涉及在统计学和遗传学，生理学和生物技术，人口生态学和遗传学方面有经验的调查人员，并期望高级人员和他们的学生，以及他们的科学，将受益于互动。