Chromosomal inversions and gene expression in Mimulus

含酸浆的染色体倒位和基因表达

• 批准号: 1940785
• 负责人: John Kelly
• 金额: $ 74.96万
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1940785&HistoricalAwards=false
• 关键词: Chromosomal; inversions; gene; expression; Mimulus

Chromosomal inversions are a type of mutation in which a section of a chromosome is flipped in orientation. This research project will provide a broad, quantitative evaluation of this important class of mutations to gene expression variation in several species of monkeyflower. It will provide essential genetic and bioinformatic training to a graduate student and a post-doctoral research scientist. The project will also extend ongoing efforts to engage Kansas high-school students in genetics research.Inversions alter the DNA sequence in a way that is fundamentally different from nucleotide substitutions (the most commonly studied kind of mutation) and have been implicated in a remarkable range of natural phenomena including adaptation to novel environments and speciation. In humans, they cause of a number of diseases and also contribute to variation in non-disease traits. However, despite countless examples, there still is no quantitative understanding for how much inversions contribute to natural genetic variation and genome evolution. This research project will provide a quantitative evaluation within a species complex of monkeyflowers (Mimulus guttatus) where many of the disparate phenomena associated with inversions have been demonstrated. The importance of inversions, relative to other mutation types causing differences in gene expression, will be estimated at three different levels of biological organization: between individuals within a population, between populations of a species, and between species. The results are expected to provide new insights into how chromosomal inversions influence gene expression, adaptation, population divergence, and speciation.This project is co-funded by the Genetic Mechanisms Program in the Division of Molecular and Cellular Biosciences and by the Evolutionary Processes Program in the Division of Environmental Biology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

染色体倒置是一种突变，在这种突变中，一段染色体的方向发生了翻转。这项研究项目将对这类重要的突变对几种猴花的基因表达差异进行广泛、定量的评估。它将为一名研究生和一名博士后研究科学家提供基本的遗传和生物信息学培训。该项目还将继续努力，让堪萨斯州的高中生参与遗传学研究。基因转换改变DNA序列的方式与核苷酸替换(最常见的一种突变)根本不同，并与一系列引人注目的自然现象有关，包括适应新的环境和物种形成。在人类中，它们会导致许多疾病，也会导致非疾病特征的变异。然而，尽管有无数的例子，但对于倒置对自然遗传变异和基因组进化的贡献有多大，仍然没有定量的了解。这项研究项目将在猴花(Mimulus Guttatus)的物种复合体中提供定量评估，其中许多与倒置相关的不同现象已被证明。相对于导致基因表达差异的其他突变类型，倒置的重要性将在三个不同的生物组织水平上进行估计：种群内个体之间、物种种群之间和物种之间。这一结果有望为染色体倒置如何影响基因表达、适应、种群分化和物种形成提供新的见解。该项目由分子和细胞生物科学部门的遗传机制计划和环境生物学部门的进化过程计划共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

The promise and deceit of genomic selection component analyses.

基因组选择成分分析的希望和欺骗。

• DOI: 10.1098/rspb.2021.1812
• 发表时间: 2021
• 期刊: Proceedings. Biological sciences
• 作者: Kelly,JohnK

The quantitative genetics of gene expression in Mimulus guttatus

• DOI: 10.1371/journal.pgen.1011072
• 发表时间: 2024-04-01
• 期刊: PLOS GENETICS
• 影响因子: 4.5
• 作者: Veltsos，Paris;Kelly，John K.
• 通讯作者: Kelly，John K.