SBIR Phase I: Integrated CMOS Microarray Disposable for Handheld Oligonulceotide Diagnostics

这项小型企业创新研究（SBIR）第一阶段的研究将导致一种新的一次性微阵列设备，结合了固体表面寡核苷酸合成与CMOS成像传感器。由于这种技术涉及荧光团/发射器和检测器之间的直接耦合，而不是成像光学系统，该方法提供了一种手段，用于小足迹仪器具有更高的灵敏度和更低的成本比目前的微阵列仪器。该方法将取代大型台式检测仪器，大型台式检测仪器是学术和较小的非实验室机构的真实的尺寸和成本障碍，这些机构需要为新兴的遗传诊断领域进行常规或现场微阵列诊断。 所提出的几何形状还将包括纳米相材料的集成，这将使硅CMOS传感器具有高背景抑制能力。随着紧凑型阅读器仪器和新型信号转导方法的成功实施，将开发用于微阵列检测和分析的新方法，用于诸如在护理点的疾病分析和在事件点的生物威胁检测等应用。这项研究工作的成果将导致微阵列一次性用品和相关阅读器仪器的发展，这些仪器比目前的仪器更便宜，尺寸更小，并且不会牺牲灵敏度或通量。这种类型的微阵列技术的发展作为一个通用平台，为不断发展的遗传诊断领域将提供增强的能力，如基因表达谱，药物基因组学和药物发现，基因测序，疾病状态分析和其他遗传诊断（如寄生虫，病原体）。

This Small Business Innovation Research (SBIR) Phase I research will lead to a novel disposable microarray device that combines solid-surface oligonucleotide synthesis with a CMOS imaging sensor. Since this technique involves direct coupling between fluorophore/emitter and the detector instead of an imaging optical system, the method provides a means for a small-footprint instrument with higher sensitivity and lower cost than present microarray instrumentation. The method will replace large benchtop detection instrumentation that are a real size and cost barrier for academic and smaller non-laboratory institutions that need to perform routine or fielded microarray diagnostics for the emerging field of genetic diagnostics. The proposed geometry will also include the integration of nanophase material that will enable high background rejection on the silicon CMOS sensor. With successful implementation of the compact reader instrumentation and novel signal transduction method, a new approach for microarray detection and analysis will be developed for applications such as disease profiling at the point-of-care and biothreat detection at the point-of-incident. The product of this research effort will lead to the development of microarray disposables, and associated reader instrumentation, that are less expensive and much smaller in size than present instrumentation and do not sacrifice sensitivity or throughput. Development of this type of microarray technology as a general purpose platform for the evolving field of genetic diagnostics will provide enhanced capabilities in areas such as gene expression profiling, pharmacogenomics and drug discovery, genetic sequencing, disease state profiling and other genetic diagnostics (e.g. parasites, pathogens).