SBIR PHASE II, TOPIC 420: INTEGRATED PLATFORM AND CONSUMABLES FOR ROBUST, SENSITIVE AND HIGH-THROUGHPUT SINGLE-CELL PROTEOMICS

SBIR 第二阶段，主题 420：用于稳健、灵敏和高通量单细胞蛋白质组学的集成平台和耗材

• 批准号: 10948319
• 负责人: RYAN KELLY
• 金额: $ 200万
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-25 至 2025-08-24
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10948319
• 关键词: SBIR; PHASE; II; TOPIC; 420

Mass spectrometry-based proteomics can now quantify up to thousands of proteins from trace samples including single cells, yet such studies require highly specialized, custom-built consumables and instrumentation, and are thus out of reach of most laboratories. Commercial solutions now exist for cell isolation and reagent dispensing at the beginning of the single-cell proteomics (SCP) workflow, and highly sensitive mass spectrometry is available at the end of the workflow, but there is a large gap in the middle comprising sample introduction, separations and ionization. Our objective is to provide robust sample-to answer workflows for non-specialist end users for SCP and other trace bioanalyses. We will develop (1) validated sample processing reagents and consumables for streamlined sample preparation; (2) high performance liquid chromatography columns specifically for SCP; (3) a robust multi-column LC platform for high throughput separations. We will achieve a depth of proteome coverage >2,500 proteins per cell and a throughput of 96 cells per day (15 min/cell). The platform will also be compatible with multiplexed workflows such as tandem mass tags for an order of magnitude higher throughput. We will thus establish an innovative platform for advancing biomedical research, biomarker discovery, diagnostics and individualized therapy.

基于质谱的蛋白质组学现在可以从包括单细胞在内的痕量样品中定量多达数千种蛋白质，但这种研究需要高度专业化的定制耗材和仪器，因此大多数实验室都无法实现。目前，在单细胞蛋白质组学（SCP）工作流程的开始阶段，已经存在用于细胞分离和试剂分配的商业解决方案，在工作流程的最后阶段，可以使用高灵敏度的质谱法，但在中间阶段存在很大差距