RUI: Flavonoid Glucosyltransferase Clones From Citrus paradisi; Function, Metabolic Impact, and Structural Characterization

RUI：来自柑橘的类黄酮葡萄糖基转移酶克隆；

• 批准号: 0614260
• 负责人: Cecilia McIntosh
• 金额: --
• 依托单位: East Tennessee State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0614260&HistoricalAwards=false
• 关键词: RUI; Flavonoid; Glucosyltransferase; Clones; Citrus

The overall research focus of this project is to study regulation of biosynthesis of specific flavonoids (a major group of phenolic metabolites) to elucidate factors that control flavonoid synthesis, metabolism, and accumulation during plant development and growth. A major emphasis area is on regulation of enzymes called glucosyltransferases (GTs) that add glucose to different classes of flavonoids resulting in production of the compounds actually found in plant tissues. Well over 100 putative secondary product GTs can be predicted from sequence analysis of various plant genomes; only a relatively small number of these gene products have been functionally characterized and the majority of these belong to a group of GTs that add glucose to flavonols and/or anthocyanins at the 3-OH position. This research uses grapefruit as the model system as this plant has been shown to have GT enzymes that act on different flavonoids. Also, some candidate clones are already available for characterization. Specific goals of this research are to test the hypotheses that the grapefruit leaf putative GT clones are secondary product GTs, specifically flavonoid GTs, and that they have properties that may make them subject to regulation at the biochemical level. In addition, studies designed to test hypotheses related to GT structure/function by determining whether or not particular structural feature(s) of these proteins are responsible for their unique properties will be initiated. Broader Impact: This research provides quality training and educational opportunities to undergraduate students and master's level graduate students at East Tennessee State University, a primarily undergraduate institution located in an economically disadvantaged region. It also provides an opportunity for a postdoctoral research associate to obtain experience and requisite skills for forming an active research program at an undergraduate institution and developing research activities in support of teaching and mentoring student research. The basic science and modern approach of this project will provide a good launching point for discussions during weekly lab meetings and journal clubs. In addition, the project personnel will be involved in outreach activities to strengthen K-12 science education.

本项目的总体研究重点是研究特定类黄酮(主要的酚类代谢物)的生物合成规律，以阐明在植物发育和生长过程中控制类黄酮合成、代谢和积累的因素。一个主要的重点领域是对被称为葡萄糖基转移酶(GTS)的酶的调节，这种酶将葡萄糖添加到不同类别的类黄酮类化合物中，从而产生实际上在植物组织中发现的化合物。通过对不同植物基因组的序列分析，可以预测超过100种可能的次生产物GT；只有相对较少的这些基因产物被功能鉴定，其中大多数属于在3-OH位置将葡萄糖添加到黄酮醇和/或花青素的GT组中。这项研究使用葡萄柚作为模型系统，因为这种植物已经被证明具有作用于不同类黄酮的GT酶。此外，一些候选克隆已经可以用于鉴定。这项研究的具体目标是检验以下假设：葡萄柚叶假定的GT克隆是副产品GT，特别是类黄酮GT，它们具有可能使它们受到生化水平调节的特性。此外，将启动旨在通过确定这些蛋白质的特定结构特征(S)是否对其独特性质负责来检验与GT结构/功能相关的假说的研究。更广泛的影响：这项研究为东田纳西州立大学的本科生和硕士研究生提供了高质量的培训和教育机会，东田纳西州立大学是一所位于经济贫困地区的主要本科院校。它还为博士后研究助理提供机会，以获得经验和必要的技能，以便在本科生机构形成积极的研究计划，并开展研究活动，以支持教学和指导学生的研究。这个项目的基础科学和现代方法将为每周实验室会议和期刊俱乐部的讨论提供一个很好的起点。此外，项目人员将参与加强K-12科学教育的外联活动。