Biophotonics: Imaging the temporal and spatial regulation of microRNA in living cells

本课题的总体目标是开发一种分子光学探针，可用于单个活细胞中MicroRNAs （miRNAs）表达水平的定量时空监测。一种新型集成光学探针将被构建，将能够检测荧光报告和猝灭在另一个。除了这种分子信标结构外，另一种光学探针将附着在该结构上，该结构将携带稳定的染料，并将作为参考点，保持不淬灭。这将有助于使用集成探针在空间和时间上对mirna进行定量测量。

0616031TsourkasThe overall goal of this proposal is to develop a molecular optical probe that can be used for quantitative spatial and temporal monitoring of MicroRNAs (miRNAs) expression levels in single living cell. A novel integrated optical probe will be constructed that will enable detection of a fluorophore reporter and a quencher at the other. In addition to this Molecular Beacon structure, another optical probe will be attached to this structure that will carry a stable dye and will function as a reference point and remains unquenched. This will facilitate the use of the integrated probe for quantitative measurements of miRNAs in both space and time.