Biophotonics: Imaging the temporal and spatial regulation of microRNA in living cells
Biophotonics: Imaging the temporal and spatial regulation of microRNA in living cells
批准号:
0616031
负责人:
Andrew Tsourkas
金额:
$35.5万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2006
资助国家:
美国
项目状态:
已结题
起止时间:
2006-09-01 至 2010-08-31
中文摘要
本提案的总体目标是开发一种分子光学探针,可用于对单个活细胞中的microRNAs(MiRNAs)表达水平进行定量的空间和时间监测。将构建一种新的集成光学探测器,该探测器将能够同时检测荧光团报告器和猝灭剂。除了这个分子信标结构外,另一个光学探头将附着在这个结构上,它将携带稳定的染料,并将作为参考点发挥作用,保持不熄灭。这将有助于使用集成探针在空间和时间上对miRNAs进行定量测量。
英文摘要
0616031TsourkasThe overall goal of this proposal is to develop a molecular optical probe that can be used for quantitative spatial and temporal monitoring of MicroRNAs (miRNAs) expression levels in single living cell. A novel integrated optical probe will be constructed that will enable detection of a fluorophore reporter and a quencher at the other. In addition to this Molecular Beacon structure, another optical probe will be attached to this structure that will carry a stable dye and will function as a reference point and remains unquenched. This will facilitate the use of the integrated probe for quantitative measurements of miRNAs in both space and time.
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CAREER: Molecular Imaging Training Program
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批准号:0953583
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项目类别:Continuing Grant
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资助金额:$40.0万
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财政年份:2010
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负责人:Andrew Tsourkas
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依托单位:
国内基金
海外基金
非小细胞肺癌Biomarker的Imaging MS研究新方法
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批准号:30672394
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项目类别:面上项目
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资助金额:30.0万元
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批准年份:2006
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负责人:陆豪杰
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依托单位: