Collaborative Proposal: In Situ Quantification of Sponge N Cycling in Coastal Ecosystems

合作提案：沿海生态系统海绵氮循环的原位定量

• 批准号: 0624406
• 负责人: Christopher Martens
• 金额: $ 64.67万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0624406&HistoricalAwards=false
• 关键词: Collaborative; Proposal; Situ; Quantification; Sponge

ABSTRACTOCE-0624406 / OCE-0624703In earlier NSF-funded investigations of nitrogen cycling in sponges, a research team at the University of North Carolina at Chapel Hill demonstrated that sponges play a major role in the N budget of coral reef and other shallow water tropical ecosystems. Their in situ measurements of whole sponge respiration, water pumping rates and chemical fluxes indicate that previous studies based upon enclosure or laboratory experiments seriously underestimate net fluxes and cannot provide data needed to establish actual net fluxes of dissolved inorganic nitrogen (DIN) and other N species. Marine sponges strongly affect N cycling in coastal environments through pumping and filtering tremendous volumes of water while their hosted microorganisms affect a wide range of N transformations, including nitrification and dissolved and particulate organic nitrogen uptake and degradation, and potentially N2 fixation and denitrification, thereby adding or subtracting bioavailable N from coastal systems. Sponge DIN flux to overlying waters at Conch Reef, Florida Keys, is larger than any other benthic N source as should be the case for all coastal systems where sponges are abundant. With additional funding under this award, the UNC team will be joined by biogeochemical colleagues at the University of Hawaii in an effort he to quantify sponge impacts on coastal N cycles by (i) using in situ methodologies to construct accurate N budgets for sponges that will identify them as nutrient sources or sinks, and (ii) investigating environmental controls over sponge mediated N transformations. The net impact of sponges on biogeochemical N cycling in coastal environments is largely unknown. This new work will be the first to use in situ methods to quantify the nitrogen cycling in sponges and the resultant fluxes of DIN and DON from sponges. The project will have a number of broader impacts. The information gained in this study, although directly applicable to a subset of sponge species, will have broad implications for nitrogen biogeochemistry in coastal ecosystems with abundant sponges. A graduate and several undergraduate students will learn in both laboratory and field settings a spectrum of state-of-the-art techniques and instrumentation firsthand and, when the results are ultimately synthesized for publication, will benefit from participation in the exchange of ideas with established researchers from diverse fields. The University of Hawaii is a designated EPSCoR institution and a federally recognized minority serving institution, thus students from underrepresented groups are likely to be included as participants in the proposed research and will benefit from education activities stemming from the results of this research. Finally, using the University of North Carolina's established connections with Earth Eco International (Phillipe Cousteau, President) and the Science Under the Sea, educational projects, significant public outreach efforts will result including live web-based videoconferences from AQUARIUS with high school and middle school students across the country.

Abstractoce-0624406 / OCE-0624703在北卡罗来纳大学教堂山北卡罗来纳大学的一个研究团队中，NSF资助的氮循环研究较早，Chapel Hill的研究小组表明，海绵中的核心礁N预算和其他浅层水上热带生态生态系统都起着重要作用。它们对整个海绵呼吸，水泵速率和化学通量的原位测量表明，基于外壳或实验室实验的先前研究严重低估了净通量，并且无法提供所需的数据来建立溶解的无机氮（DIN）和其他N物种的实际净通量。海洋海绵在沿海环境中通过抽水和过滤大量水强烈影响n循环，而它们的宿主微生物会影响广泛的N转化，包括硝化和溶解和颗粒的有机氮的吸收和脱位，并潜在地固定n2固定和潜在地添加了沿海或分类的n2固定系统。佛罗里达钥匙海岸礁的海绵din通量比所有其他底栖n来源都要大，因为对于所有丰富海绵的沿海系统都应该如此。 With additional funding under this award, the UNC team will be joined by biogeochemical colleagues at the University of Hawaii in an effort he to quantify sponge impacts on coastal N cycles by (i) using in situ methodologies to construct accurate N budgets for sponges that will identify them as nutrient sources or sinks, and (ii) investigating environmental controls over sponge mediated N transformations.海绵对沿海环境中生物地球化学N循环的净影响在很大程度上是未知的。这项新作品将是第一个使用原位方法来量化海绵中的氮循环以及来自海绵的DIN和DON的最终通量。该项目将产生更广泛的影响。在这项研究中获得的信息虽然直接适用于海绵物种的一部分，但对沿海生态系统中具有丰富海绵的氮生物地球化学将具有广泛的影响。一位毕业生和几个本科生将在实验室和现场环境中学习一系列最先进的技术和仪器，而当最终合成结果以出版的结果时，将受益于参与与既定领域的既定研究人员的思想交流。夏威夷大学是一家指定的EPSCOR机构，也是一家联邦认可的少数民族服务机构，因此，来自代表性不足的群体的学生很可能被包括在拟议的研究中作为参与者，并将受益于本研究结果所源于的教育活动。最后，利用北卡罗来纳大学与地球生态国际（Phillipe Cousteau，总裁）和海底科学的建立联系，教育项目，包括Aquarius的高中和中学生的基于Web的现场视频会议，包括实时的公共外展工作。