`Molecular Mechanisms of pheromone Perception: Role of Pheromone Binding Protein

信息素感知的分子机制：信息素结合蛋白的作用

• 批准号: 0628064
• 负责人: Smita Mohanty
• 金额: $ 62.42万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0628064&HistoricalAwards=false
• 关键词: Molecular; Mechanisms; pheromone; Perception; Role

The ability to detect and respond to chemical stimuli is a fundamental behavior of all organisms. Lepidoptera male moths have an exquisitely sensitive olfactory system that is capable of perceiving airborne pheromone molecules released by females and responding to them over great distances. They are capable of distinguishing between closely related pheromones of different species. Pheromone binding proteins play an important role in olfaction. They are carrier proteins that transport the volatile hydrophobic pheromone molecules across the aqueous lymph to the membrane-bound olfactory receptors. Interaction of pheromone and pheromone binding protein is the first step in the sequential events that leads to the activation of the receptor. Despite over 50 % sequence identity pheromone binding proteins from different moth species have different substrate specificity. The overall goal of this project is to understand the role of pheromone binding protein in the process of recognition. This project utilizes biochemistry, molecular biology, and structural biology to test the hypothesis that pheromone specificity is encoded in the pheromone binding proteins. Knowledge gained from these studies may have significance to insect control, through pheromone based integrated pest management programs to control agricultural pests and also those who are carrier of vector borne diseases. This project will contribute to Stony Brook University through "Women in Science and Engineering program provide training opportunities for high school, undergraduate and graduate students, particularly for women and under-represented groups.

探测和响应化学刺激的能力是所有生物体的基本行为。鳞翅目雄蛾有一种极其灵敏的嗅觉系统，能够感知雌蛾释放的空气中的信息素分子，并在很远的距离对其做出反应。它们能够区分不同物种密切相关的信息素。信息素结合蛋白在嗅觉中起着重要作用。它们是载体蛋白，将挥发性疏水信息素分子通过水淋巴输送到膜结合的嗅觉感受器。信息素和信息素结合蛋白的相互作用是导致受体激活的一系列事件的第一步。尽管序列同源性超过50%，但不同蛾种信息素结合蛋白具有不同的底物专一性。这个项目的总体目标是了解信息素结合蛋白在识别过程中的作用。这个项目利用生物化学、分子生物学和结构生物学来验证信息素特异性编码在信息素结合蛋白中的假设。从这些研究中获得的知识可能对通过以信息素为基础的害虫综合管理计划来控制农业害虫和那些媒介传播疾病的人的害虫控制具有重要意义。该项目将通过“妇女参与科学和工程方案”为石溪大学做出贡献，该方案为高中生、本科生和研究生提供培训机会，特别是为女性和代表性不足群体提供培训机会。