MRI: Acquisition of the First 800 MHz NMR Spectrometer with a Cryogenically Cooled Probe in the State of Oklahoma for Interdisciplinary Research and Training

MRI：在俄克拉荷马州采购第一台带有低温冷却探头的 800 MHz NMR 波谱仪，用于跨学科研究和培训

• 批准号: 1726397
• 负责人: Smita Mohanty
• 金额: $ 228.86万
• 依托单位: Oklahoma State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726397&HistoricalAwards=false
• 关键词: MRI; Acquisition; First; 800; MHz

With support from the Major Research Instrumentation (MRI) Program, an award has been made to Oklahoma State University for the acquisition of a 800 MHz NMR instrument with a cryoprobe. This instrument will be housed in the Oklahoma Statewide Shared NMR Facility to ensure that it is accessible to researchers in Oklahoma and the surrounding region. The new spectrometer will support the training of the next generation of researchers in modern state-of-the-art NMR techniques. Oklahoma State University is aggressively pursing hands-on undergraduate research training through courses, and independent research. In addition, programs in the Chemistry Department such as "Research Experience for Undergraduate Students (REU)" supported by National Science Foundation allows recruitment of undergraduate students across the country. Minority undergraduate students are recruited for research through various undergraduate programs such as the Oklahoma Louis Stokes Alliance for Minority Participation (OK-LSAMP). Nuclear Magnetic Resonance (NMR) spectroscopy is one of the most powerful scientific techniques that is available to scientist for the elucidation of the structure of important molecules. Projects supported by this new instrument span the range from Chemical Biology to protein structure and function, protein dynamics, RNA/DNA structure, complex carbohydrate structure, design of novel polymer, and small complex molecules characterization during the process of drug design. The new NMR is an essential resource for researchers in Chemical Biology, Structural Biology, Biochemistry, Molecular Biology, Microbiology, and Polymer Science across Oklahoma and the surrounding region. The results of these research and teaching efforts will be disseminated through active participation of students and faculty at scientific conferences, symposia and workshops, peer-reviewed publications and the deposition of atomic coordinates of biomolecules to the Protein Data Bank (PDB).

在主要研究仪器 (MRI) 计划的支持下，俄克拉荷马州立大学获得了购买带有冷冻探头的 800 MHz NMR 仪器的奖励。 该仪器将安装在俄克拉荷马州全州共享核磁共振设施中，以确保俄克拉荷马州及周边地区的研究人员可以使用它。新型光谱仪将支持对下一代研究人员进行现代最先进核磁共振技术的培训。俄克拉荷马州立大学正在通过课程和独立研究积极追求本科生的实践研究培训。此外，美国国家科学基金会支持的化学系“本科生研究体验（REU）”等项目允许在全国范围内招收本科生。通过各种本科项目招募少数族裔本科生进行研究，例如俄克拉荷马州路易斯斯托克斯少数族裔参与联盟（OK-LSAMP）。核磁共振 (NMR) 光谱是科学家可用于阐明重要分子结构的最强大的科学技术之一。该新仪器支持的项目涵盖从化学生物学到蛋白质结构和功能、蛋白质动力学、RNA/DNA 结构、复杂碳水化合物结构、新型聚合物设计以及药物设计过程中复杂小分子表征的范围。新的 NMR 是俄克拉荷马州及周边地区化学生物学、结构生物学、生物化学、分子生物学、微生物学和聚合物科学研究人员的重要资源。这些研究和教学工作的成果将通过学生和教师积极参与科学会议、专题讨论会和讲习班、同行评审出版物以及将生物分子原子坐标存入蛋白质数据库（PDB）来传播。

项目成果

Advancing viral RNA structure prediction: measuring the thermodynamics of pyrimidine-rich internal loops

• DOI: 10.1261/rna.059865.116
• 发表时间: 2017-05-01
• 期刊: RNA
• 影响因子: 4.5
• 作者: Phan, Andy;Mailey, Katherine;Schroeder, Susan J.
• 通讯作者: Schroeder, Susan J.

NMR and MD simulations reveal the impact of the V23D mutation on the function of yeast oligosaccharyltransferase subunit Ost4

NMR 和 MD 模拟揭示了 V23D 突变对酵母寡糖转移酶亚基 Ost4 功能的影响

• DOI: 10.1093/glycob/cwab002
• 发表时间: 2021
• 期刊: Glycobiology
• 影响因子: 4.3
• 作者: Chaudhary, Bharat P;Zoetewey, David L;McCullagh, Martin J;Mohanty, Smita
• 通讯作者: Mohanty, Smita

Backbone and side chain NMR assignments and secondary structure calculation of the pheromone binding protein3 of Ostrinia nubilalis, an agricultural pest

• DOI: 10.1007/s12104-023-10145-3
• 发表时间: 2023-07-27
• 期刊: BIOMOLECULAR NMR ASSIGNMENTS
• 影响因子: 0.9
• 作者: Al-Danoon，Omar;Mohanty，Smita
• 通讯作者: Mohanty，Smita

1H, 13C, and 15N resonance assignment and secondary structure of the pheromone-binding protein2 from the agricultural pest Ostrinia furnacalis (OfurPBP2)

• DOI: 10.1007/s12104-020-09930-1
• 发表时间: 2020-04-01
• 期刊: BIOMOLECULAR NMR ASSIGNMENTS
• 影响因子: 0.9
• 作者: Dahal, Salik R.;Lewellen, Jacob L.;Mohanty, Smita
• 通讯作者: Mohanty, Smita

Ostrinia furnacalis PBP2 solution NMR structure: Insight into ligand binding and release mechanisms

• DOI: 10.1002/pro.4438
• 发表时间: 2022-09
• 期刊: Protein Science
• 影响因子: 8
• 作者: S. R. Dahal;Jacob L Lewellen;Shine Ayyappan;B. Chaudhary;Viswanath Nukala;Smita Mohanty