GFP as a Tool for Multi-representational Visual Translation

GFP 作为多表征视觉翻译工具

• 批准号: 0633618
• 负责人: Sarah Wyatt
• 金额: $ 14万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0633618&HistoricalAwards=false
• 关键词: GFP; Tool; Multi; representational; Visual

Life Science Biological (61) This project is developing a multi-representational visual learning environment for Animal Cell Biology and Anatomy of Plant Development laboratories using green fluorescent protein (GFP) as a marker for gene and protein expression in strains of Arabidopsis and C. elegans from the research communities. Four faculty members from Biology and Education are developing the new courses to combine descriptive aspects of plant anatomy and cell biology with the modern molecular techniques needed for analysis of gene expression in living organisms. Gene and protein expression at the sub-cellular, cellular, and tissue level are emphasized in order to help students acquire rich contextual information using current research technologies. As part of these combined lab and lecture courses, students work on long-term continuous projects, hold frequent collaborative discussions, and present their work to one another. The courses promote students' development into skilled observers who can translate between their own observations and the products from the larger research community. Microscopy is the basis for these interactive laboratory experiences at both introductory (sophomore) and advanced (senior) levels where students conduct cutting edge, inquiry-based, cellular and molecular experiments. Evaluation of the results of this project will indicate to what degree research with live transgenic model organisms that express a GFP helps students interpret professional research materials, including illustrations, diagrams, and images. Information on the most useful GFP strains for subcellular and cellular identifications is disseminated through presentations at cell biology, plant biology, and instructional technology meetings and to the Arabidopsis and C. elegans research communities.

生命科学生物学(61)这个项目正在为植物发育实验室的动物细胞生物学和解剖学开发一个多代表性的可视化学习环境，使用绿色荧光蛋白(GFP)作为来自研究社区的拟南芥和线虫品系中基因和蛋白质表达的标记。来自生物学和教育学的四名教员正在开发新课程，将植物解剖学和细胞生物学的描述方面与分析活生物体基因表达所需的现代分子技术结合起来。强调亚细胞、细胞和组织水平的基因和蛋白质表达，以帮助学生利用当前的研究技术获得丰富的背景信息。作为这些实验和讲座课程的一部分，学生们在长期持续的项目中工作，经常进行协作讨论，并相互展示他们的工作。这些课程促进学生发展成为熟练的观察者，能够在他们自己的观察和更大的研究社区的产品之间进行转换。显微镜是这些互动实验室体验的基础，在初级(二年级)和高级(高级)水平上，学生进行尖端的、基于探究的、细胞和分子实验。对该项目结果的评估将表明，对表达绿色荧光蛋白的活转基因模型生物的研究在多大程度上有助于学生解释专业研究材料，包括插图、图表和图像。关于用于亚细胞和细胞鉴定的最有用的绿色荧光蛋白菌株的信息通过在细胞生物学、植物生物学和教学技术会议上的陈述以及向拟南芥和线虫的研究社区传播。