SGER: The Development of an in PlantaTransformation Technology to Facilitate Functional Genomics in Wheat

SGER：开发植物转化技术以促进小麦功能基因组学

• 批准号: 0638421
• 负责人: Janice Zale
• 金额: --
• 依托单位: University of Tennessee Institute of Agriculture
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0638421&HistoricalAwards=false
• 关键词: SGER; Development; PlantaTransformation; Technology; Facilitate

PI: Janice M. Zale (University of Tennessee)CoPI: Katrien M. Devos (University of Georgia)Current wheat transformation technologies are technically difficult, time-consuming, labor intensive, expensive, and rely upon a tissue culture phase. Furthermore, there are no transformation technologies that facilitate testing of candidate genes for small or large scale functional genomics. Thus, there is an urgent need for the development of a high throughput transformation technology, particularly in recalcitrant crop species such as wheat. The goal of this project is to address this need and develop an efficient in planta transformation protocol for use in wheat similar to that used for the model plant Arabidopsis thaliana. The specific objectives are to (1) develop and optimize a standard in planta protocol using the wheat line Crocus and generate T-DNA tagged wheat transformants and (2) sequence and map the T-DNA insertion sites of 12 independent Crocus transformants to validate that the transformants are genuine, stable, and as a first step in producing T-DNA tagged lines.Broader Impacts: The development of such a protocol would have a tremendous impact for functional genomics in wheat and potentially in other cereals. In addition, the project will provide for training of undergraduate and graduate students and for the broad dissemination of research results in papers, seminars, and conference presentations.

PI：Janice M. Zale（田纳西大学）CoPI：Katrien M. Devos（佐治亚大学）当前的小麦转化技术技术上困难、耗时、劳动密集、昂贵，并且依赖于组织培养阶段。 此外，没有转化技术可以促进小规模或大规模功能基因组学候选基因的测试。因此，迫切需要开发高通量转化技术，特别是对于小麦等顽固作物物种。 该项目的目标是满足这一需求，并开发一种用于小麦的高效植物转化方案，类似于用于模式植物拟南芥的方案。 具体目标是 (1) 使用小麦品系 Crocus 开发和优化植物方案标准，并生成 T-DNA 标记的小麦转化体，以及 (2) 对 12 个独立的番红花转化体的 T-DNA 插入位点进行测序和作图，以验证转化体是真实的、稳定的，并作为生产 T-DNA 标记品系的第一步。 更广泛的影响：此类方案的开发将对功能基因组学产生巨大影响 存在于小麦中，也可能存在于其他谷物中。 此外，该项目还将为本科生和研究生提供培训，并以论文、研讨会和会议演示形式广泛传播研究成果。