Regulation of Asexual Development in Aspergillus

曲霉无性发育的调控

• 批准号: 0640067
• 负责人: Jaehyuk Yu
• 金额: $ 40.5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0640067&HistoricalAwards=false
• 关键词: Regulation; Asexual; Development; Aspergillus

PI: Yu, JaehyukProposal #: IOB-0640067Title: Regulation of Asexual Development in Aspergillus A large number of fungi (molds) produce spores as the main means of survival and propagation. In pathogenic fungi, these spores are also the primary agent for infection of plants, animals and humans. Despite the significant impact of fungal spores in everyday life, the mechanisms underlying the initiation and the completion of spore formation are largely unknown. To better understand the regulation of spore formation two genetic screens were carried out employing the model filamentous fungus Aspergillus nidulans and two key genes controlling sporulation were identified. Characterization of these genes revealed that the beginning and the end of asexual spore formation require balanced activities of various positive and negative regulators. The genetically programmed instruction for the regulation of spore formation may explain how a fungus governs vegetative growth and reproduction appropriately. In this project, genetic, molecular biological, genomic and biochemical studies will be carried out to further investigate the roles of these crucial regulatory genes and to better understand the mechanisms underlying formation and maturation of fungal spores. Results of this project will advance the understanding of the mechanisms controlling the novel and poorly understood asexual development in fungi. The project's broader impacts include using the model fungus A. nidulans as an effective and accessible system for teaching basic and difficult genetic concepts. In addition, understanding the mechanisms controlling spore formation in the model fungus will potentially lead to the development of new tools for controlling beneficial or detrimental activities of other industrially, medically or agriculturally important fungi.

主要研究者：Yu，Jaehyuk提案编号：IOB-0640067题目：曲霉属中无性发育的调节大量真菌（霉菌）产生孢子作为生存和繁殖的主要手段。在病原真菌中，这些孢子也是植物、动物和人类感染的主要媒介。尽管真菌孢子在日常生活中的重要影响，孢子形成的启动和完成的机制在很大程度上是未知的。为了更好地理解孢子形成的调控，采用模式丝状真菌构巢曲霉进行了两次遗传筛选，并鉴定了控制孢子形成的两个关键基因。这些基因的特性表明，开始和结束的无性孢子形成需要各种积极和消极的调节平衡的活动。孢子形成的遗传程序指令可以解释真菌如何适当地控制营养生长和繁殖。本项目将通过遗传学、分子生物学、基因组学和生物化学研究，进一步探讨这些关键调控基因的作用，并更好地了解真菌孢子形成和成熟的机制。该项目的结果将促进对控制真菌中新颖且知之甚少的无性发育的机制的理解。该项目更广泛的影响包括使用模式真菌A。nidulans是教授基本和困难遗传概念的有效和可访问的系统。此外，了解控制模式真菌中孢子形成的机制将潜在地导致用于控制其他工业、医学或农业上重要的真菌的有益或有害活性的新工具的开发。