Gene Regulation and Control of Chromatin Structure by Abf1 and Rap1

Abf1 和 Rap1 对染色质结构的基因调控

• 批准号: 0641776
• 负责人: Randall Morse
• 金额: $ 45.1万
• 依托单位: Health Research Incorporated/New York State Department of Health
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0641776&HistoricalAwards=false
• 关键词: Gene; Regulation; Control; Chromatin; Structure

Rap1 and Abf1 are General Regulatory Factors (GRFs) that function in transcriptional activation and repression, replication, and telomere structure in yeast. These two GRFs share an ability to create a local region of open chromatin which can facilitate binding of other activators to gene promoters and thus contribute to gene activation. However, these two GRFs also differ in some respects. Most dramatically, continued binding of Rap1 is required to facilitate ongoing transcriptional activation, whereas loss of Abf1 binding (using an abf1-1 ts mutant) does not result in decreased transcription. Thus, at least some promoters retain a "memory" of Abf1 binding. In this project, new insight into the mechanism by which Rap1 and Abf1 contribute to transcriptional activation and perturb local chromatin structure, and into the basis of this "memory effect," will be obtained using a combination of biochemical, genetic, and genomic approaches.Gene activation depends on binding of specific transcription factors to gene promoters. In this project, two such factors, Abf1 and Rap1, that operate as "General Regulatory Factors" by assisting in activation of many genes in yeast will be investigated. The studies will include biochemical, genetic and genomic approaches to understanding the mechanism by which these two factors contribute to transcriptional activation, including their effects on the packaging of gene promoters into chromatin. The results of this work will provide new information that is likely to apply to all eukaryotes, as the transcription machinery is highly conserved. These investigations will include participation by undergraduates conducting summer research, and will yield genomic data that will be made available to the scientific community.

RAP1和ABF1是酵母中转录激活，复制和端粒结构的一般调节因素（GRF）。 这两个GRF具有创建开放染色质的局部区域的能力，该区域可以促进其他激活剂与基因启动子的结合，从而有助于基因激活。但是，这两个GRF在某些方面也有所不同。 大多数急剧，需要RAP1的持续结合才能促进持续的转录激活，而ABF1结合的丧失（使用ABF1-1 TS突变体）并不导致转录减少。因此，至少有些启动子保留了ABF1结合的“记忆”。 在该项目中，将对RAP1和ABF1有助于转录激活和诱饵局部染色质结构的机制进行新的见解，并将其用于这种“记忆效应”的基础，将使用生物化学，遗传和基因组方法的组合来获得。Gene激活取决于将特定转录与Gene促进者的结合。 在这个项目中，将研究两个这样的因素ABF1和RAP1作为“一般调节因素”通过协助激活酵母中的许多基因的作用。 研究将包括生化，遗传和基因组方法，以理解这两个因素导致转录激活的机制，包括它们对基因启动子包装中染色质的影响。 这项工作的结果将提供可能适用于所有真核生物的新信息，因为转录机械是高度保守的。 这些调查将包括进行夏季研究的大学生的参与，并产生将为科学界提供的基因组数据。