CCaMK-Mediated Changes During Bacterial and Fungal Symbioses

细菌和真菌共生过程中 CCaMK 介导的变化

• 批准号: 0642146
• 负责人: B. Poovaiah
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-15 至 2011-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0642146&HistoricalAwards=false
• 关键词: CCaMK; Mediated; Changes; During; Bacterial

A Ca2+/calmodulin-dependent protein kinase (CCaMK) has been implicated as a key player in the signal transduction cascade that follows perception of nitrogen fixing bacterial symbionts by plant roots and leads to the establishment of nitrogen-fixing root nodules. This project seeks to determine the role of this kinase in roots as signals from potential symbionts are received and transduced to downstream effectors involved in nodule formation. CCaMK, a multi-functional protein, is characterized by the presence of a kinase domain, an autoinhibitory domain, a calmodulin-binding domain and a neural visinin-like Ca2+-binding domain in a single polypeptide. CCaMK undergoes two steps of Ca2+ regulation, first directly through its Ca2+-binding domain and then indirectly through its Ca2+/calmodulin-binding domain. Recent investigations have revealed that CCaMK plays critical roles in both bacterial and fungal symbioses. During bacterial interactions which lead to nitrogen fixation, CCaMK acts immediately downstream of Ca2+ oscillations triggered in root hair cells by the bacterially produced signaling molecule, Nod factor. The immediate goal of this investigation is to study how the structural changes of CCaMK control bacterial invasion and the resulting symbiotic relationship. Furthermore, the project will also seek to identify and characterize CCaMK's potential downstream interaction partner(s). Genetic studies indicate that CCaMK is at a critical juncture of a branch point in the signaling pathway which mediates the interaction of plants with their symbionts: one branch leads to nodulation and the other to mycorrhizal infection. Hence, this project may lead to new insights into fungal as well as bacterial symbioses. This project will use genetic, biochemical and cellular approaches to address a seminal question in the area of plant:microbe interactions. In addition to its agricultural and ecological significance the broader impacts of the project include training undergraduates, graduate students and postdocs. Training of minorities and women who are interested in science will be emphasized. On-site participants will experience the atmosphere of an active biotechnology research laboratory. To reach a wider audience, the WSU team will also use the Washington State Higher Education Telecommunication System and organize onsite workshops.

Ca 2 +/钙调素依赖性蛋白激酶（CCaMK）在植物根系感知固氮细菌共生体并导致固氮根瘤建立的信号转导级联中起关键作用。 该项目旨在确定这种激酶在根中的作用，因为来自潜在共生体的信号被接收并转导到参与根瘤形成的下游效应子。 CCaMK是一种多功能蛋白，其特征在于在单个多肽中存在激酶结构域、自抑制结构域、钙调蛋白结合结构域和神经视蛋白样Ca 2+结合结构域。 CCaMK经历两个步骤的Ca 2+调节，首先直接通过其Ca 2+结合结构域，然后间接通过其Ca 2 +/钙调素结合结构域。 最近的研究表明，CCaMK在细菌和真菌的共生中起着关键作用。 在细菌的相互作用，导致固氮，CCaMK的行为直接下游的Ca 2+振荡触发根毛细胞由细菌产生的信号分子，结瘤因子。 本研究的直接目标是研究CCaMK的结构变化如何控制细菌入侵以及由此产生的共生关系。 此外，该项目还将寻求识别和表征CCaMK的潜在下游相互作用伙伴。 遗传学研究表明，CCaMK处于介导植物与其共生体相互作用的信号传导途径中的一个分支点的关键节点：一个分支导致菌根化，另一个导致菌根感染。 因此，该项目可能会导致对真菌和细菌共生的新见解。 该项目将使用遗传，生物化学和细胞方法来解决植物领域的一个开创性问题：微生物相互作用。 除了其农业和生态意义外，该项目更广泛的影响包括培训本科生、研究生和博士后。 将强调对少数民族和对科学感兴趣的妇女的培训。 现场参与者将体验一个活跃的生物技术研究实验室的气氛。 为了接触到更广泛的受众，WSU团队还将使用华盛顿州高等教育电信系统，并组织现场研讨会。