Calcium/Calmodulin-mediated Signaling During Plant-microbe Interactions

钙/钙调素介导的信号传导在植物生长发育以及对生物和非生物刺激的响应中起着关键作用。 该项目是PI实验室最近在钙/钙调素介导的植物生长调节、细菌和真菌共生过程中植物-微生物相互作用以及植物免疫/防御反应背景下识别、表征和操纵钙/钙调素结合蛋白的工作的后续。 AtSR 1/CaMTA 3和CCaMK/DMI 3是钙/钙调素调节蛋白的两个例子，在植物-微生物相互作用中发挥核心作用。 AtSR 1/CaMTA 3作为负调节因子，控制水杨酸（一种阿司匹林样化合物）介导的植物免疫/防御。 CCaMK/DMI 3在控制植物中细菌和真菌共生中起着核心作用。 尽管最近在理解钙/钙调素如何参与AtSR 1/CaMTA 3和CCaMK/DMI 3的调节方面取得了进展，但许多潜在的基本问题尚不清楚，需要进一步研究。 本研究将采用多学科的方法来剖析AtSR 1/CaMTA 3和CCaMK/DMI 3介导的信号转导。 具体而言，本研究将确定相互作用的伙伴，并研究其功能的意义，在植物-微生物相互作用。 这项研究可能对未来的农业和环境问题产生重大影响。 对植物防御的研究可能导致抗病作物的生产，而对植物-微生物共生的研究可能导致生产增加氮和磷吸收的作物，从而减少对化石燃料肥料的依赖。 更广泛的影响包括培养对科学事业感兴趣的本科生、研究生和博士后研究人员。

Calcium/calmodulin-mediated signaling plays critical roles in plant growth and development as well as in response to biotic and abiotic stimuli. This project follows up on recent work from the PI's laboratory on identifying, characterizing and manipulating calcium/calmodulin-binding proteins in the context of calcium/calmodulin-mediated regulation of plant growth, plant-microbe interactions during bacterial and fungal symbioses and plant immune/defense responses. AtSR1/CaMTA3 and CCaMK/DMI3 are two examples of calcium/calmodulin-regulated proteins that play central roles in plant-microbe interactions. AtSR1/CaMTA3 acts as a negative regulator and controls plant immunity/defense mediated by salicylic acid (an aspirin-like compound). CCaMK/DMI3 plays a central role in controlling bacterial and fungal symbioses in plants. Despite recent advances in the understanding how calcium/calmodulin is involved in the regulation of AtSR1/CaMTA3 and CCaMK/DMI3, many underlying basic questions are not clear and warrant further research. This investigation will use multi-disciplinary approaches to dissect the AtSR1/CaMTA3 and the CCaMK/DMI3-mediated signaling. Specifically, this investigation will identify interaction partners and study their functional significance during plant-microbe interactions. This research could have major impacts on future agricultural and environmental issues. Studies on plant defense could lead to the production of disease-resistant crop plants while studies on plant-microbe symbioses could lead to the production of crop plants with increased nitrogen and phosphorus acquisition, thereby reducing the dependence on fossil fuel-based fertilizers. The broader impacts include training of undergraduates, graduate students and postdoctoral researchers who are interested in careers in science.