Simple Methods for Oligonucleotide Purification

该项目的重点是开发适合大规模纯化寡核苷酸的方法。 有两个具体目的：（1）开发使用可聚合的加帽剂来加帽失败序列的寡核苷酸纯化方法和（2）开发使用可聚合的亚磷酰胺来官能化全长序列的5 '末端的寡核苷酸纯化方法。 第一种方法设计用于纯化大量的5-至20-聚体寡核苷酸，而第二种方法设计用于纯化20-至100-聚体。有了这个奖项，有机和高分子化学计划正在支持密歇根理工大学化学系方世跃教授的研究。 方教授的研究工作围绕着开发用于大规模纯化寡核苷酸的简单方法。 方教授的项目的成功完成可以使寡核苷酸变得容易获得，从而有助于开发使用寡核苷酸的实用治疗剂。

This project is focused on the development of methods suitable for the large scale purification of oligonucleotides. There are two specific objectives: (1) to develop an oligonucleotide purification method using a polymerizable capping agent to cap failure sequences and (2) to develop an oligonucleotide purification method that uses a polymerizable phosphoramidite to functionalize the 5'-end of full-length sequences. The first approach is designed for the purification of large quantities of 5- to 20-mers oligonucleotides while the second one is designed for the purification of 20- to 100-mers. With this award, the Organic and Macromolecular Chemistry Program is supporting the research of Professor Shiyue Fang, of the Department of Chemistry at Michigan Technological University. Professor Fang's research efforts revolve around the development of simple methods for the large scale purification of oligonucleotides. Successful accomplishment of Professor Fang's project could make oligonucleotides readily available, thus assisting the development of practical therapeutic agents using oligonucleotides.