IDBR: Development of an Advanced Quadrupole Ion Trap Mass Spectrometer for Proteomics

IDBR：开发用于蛋白质组学的先进四极离子阱质谱仪

• 批准号: 0649757
• 负责人: Glen Jackson
• 金额: $ 22.82万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649757&HistoricalAwards=false
• 关键词: IDBR; Development; Advanced; Quadrupole; Ion

This award is to a beginning investigator for developing an improved quadrupole ion trap mass spectrometer for proteomics. A new electrodynamic approach will be used for fragmenting biological ions in quadrupole ion traps (QIT). This new approach has four potential advantages over conventional methods: 1) the ability to acquire more tandem mass spectra per second than commercial instruments, thus enabling more complete proteome coverage when combined with bottom-up separation systems; 2) the ability to probe higher energy fragmentation pathways, which will offer complementary sequence coverage to conventional methods; 3) the ability to recapture and mass-analyze low mass product ions, which is highly beneficial for sequencing peptides and performing quantitative proteomics; 4) the ability to increase the size of precursor ions that can be fragmented in QITs, thereby expanding capabilities to top-down proteomics. The new instrument will utilize a method of collisional activation, called dynamic collision-induced dissociation (DCID), whereby precursor ions can be selectively fragmented during the mass acquisition scan. The majority of this work will involve software development in order to run an ion trap in the non-conventional method required to accomplish DCID. Programs will also be written to perform sequence-looped experiments that are capable of examining or optimizing the influence of different experimental parameters in an automated manner. To help streamline the implementation of this development into future instruments, considerable effort will be placed on determining a set or sets of optimal experimental parameters that will be universally applicable to a wide range of precursor ions. Because the application of this novel fragmentation method does not require any hardware modifications, developments made through this work will be readily available for implementation into existing or new quadrupole or linear ion traps, and could therefore find immediate use in diverse biological applications. Mass spectrometry is a central technology for proteomics. Proteomics, the large-scale study of proteins, is vital to understanding organisms. In addition to this scientific benefit, the award will have valuable educational benefits. Undergraduate and graduate students will learn first-hand about instrumentation development and the various methods of mass spectrometry that are used in biological research. The award will support two female graduate students and also undergraduate students during the summer quarters. Students will learn technical and engineering skills related to instrumentation development that are uncommon and highly sought-after in the instrumentation industry.

这个奖项是给一名开发改进的蛋白质组学四极离子阱质谱仪的初级研究者。一种新的电动力学方法将被用于四极离子阱（QIT）中生物离子的碎片化。与传统方法相比，这种新方法具有四个潜在优势：1)与商用仪器相比，每秒能够获得更多的串联质谱，从而与自下而上的分离系统相结合时能够更完整地覆盖蛋白质组；2)探测更高能量碎片路径的能力，这将为传统方法提供互补的序列覆盖；3)能够重新捕获和大量分析低质量产物离子，这对肽测序和定量蛋白质组学非常有益；4)增加qit中可破碎的前体离子大小的能力，从而扩大自上而下蛋白质组学的能力。新仪器将利用一种称为动态碰撞诱导离解（DCID）的碰撞激活方法，通过这种方法，前体离子可以在质量采集扫描期间选择性地破碎。这项工作的大部分将涉及软件开发，以便在完成DCID所需的非常规方法中运行离子阱。还将编写程序来执行序列循环实验，这些实验能够以自动化的方式检查或优化不同实验参数的影响。为了帮助将这一发展简化为未来仪器的实施，将付出相当大的努力来确定一组或几组最佳实验参数，这些参数将普遍适用于广泛的前体离子。由于这种新型碎片化方法的应用不需要任何硬件修改，因此通过这项工作所取得的进展将很容易实现到现有或新的四极或线性离子阱中，因此可以在各种生物应用中立即使用。质谱法是蛋白质组学的核心技术。蛋白质组学是对蛋白质的大规模研究，对了解生物体至关重要。除了这一科学效益，该奖项还将具有宝贵的教育效益。本科生和研究生将学习第一手的仪器开发和质谱的各种方法，用于生物研究。该奖项将在夏季学期资助两名女研究生和两名本科生。学生将学习与仪器仪表开发相关的技术和工程技能，这些技能在仪器仪表行业中是不常见的，也是非常受欢迎的。