Cometabolic Degradation of Estrogen Compounds in Activated Sludge Treatment Processes

CBET-0652109H。大卫Stensel华盛顿大学在活性污泥处理过程中雌激素化合物的共代谢降解雌激素是一组重要的污染物，可能导致淡水中鱼类种群数量减少，这些淡水接收来自城市污水处理设施的排放。需要关于其生物降解的信息，以设计有效的处理方法。本研究项目将进行实验室研究，以了解硝化废水系统中雌激素的生物降解机制和影响雌激素生物降解率的因素，并提出提高雌激素降解率的方法。初步研究将包括利用纯培养的铵氧化细菌（AOB），N. europaea、亚硝化螺旋菌属和寡养亚硝化单胞菌属。EE 2降解机制的研究将增加对各种酶促反应的作用的理解，并评估降解产物。将获得工艺模型中雌激素化合物的生物降解率数据，该模型包括雌激素、铵和溶解氧浓度的影响，以及ng/L至ug/L范围内的雌激素混合物的影响。雌激素降解/硝化模型将用于设计优化的活性污泥工艺。其他共代谢生物，如甲烷和苯酚氧化细菌的雌激素降解能力也将进行研究。

CBET-0652109H. David StenselUniversity of WashingtonCometabolic Degradation of Estrogen Compounds in Activated Sludge Treatment ProcessesEstrogens constitute a group of important contaminants that may contribute to low fish populations in freshwaters receiving discharges from municipal wastewater treatment facilities. Information on their biodegradation is needed to design effective treatment methods. This research project will conduct laboratory studies to understand the biodegradation mechanism for estrogen and the factors that affect estrogen biodegradation rates in nitrifying wastewater systems and advance methods to enhance estrogen degradation. Initial studies will involve fundamental investigations of EE2 degradation of the synthetic birth control pharmaceutical, 17a-ethynyl estradiol (EE2), by pure cultures of ammonium-oxidizing bacteria (AOB), N. europaea, Nitrosospira, and Nitrosomonas oligotropha. Studies of EE2degradation mechanisms will increase understanding of the role of various enzymatic reactions and assess degradation products. Biodegradation rate data will be obtained for the estrogen compounds for a process model that includes the effects of estrogen, ammonium and dissolved oxygen concentration, and effects of mixtures of estrogens in the ng/L to ug/L range. The estrogen degradation/nitrification model will be used to design optimized activated sludge processes. The estrogen degradation ability of other cometabolic organisms, such as methane- and phenol-oxidizing bacteria also will be investigated.