Genomic Resources for the Study of Cotton-Reniform Nematode Interactions

Pi：Ramesh Kantty(阿拉巴马农业与机械大学)Copis：Elica S.Moss(阿拉巴马农业与机械大学)和Katheryn S.Lawrence(奥本大学，亚奖获得者)合作者：Yong-李晓(J.Craig Venter Institute，Inc.，亚奖获得者)和Bruce Roe(俄克拉荷马大学诺曼分校，亚奖获得者)棉花是世界上种植最广泛的天然纤维。然而，棉花需要集约化管理，以控制害虫和病原体。在不久的将来，仅线虫对棉花造成的损失就可能达到10亿美元；到目前为止，还没有棉花品种对肾形线虫产生抗性。肾形线虫与棉花根的相互作用在生化、形态和生理水平上引起复杂的植物反应，而这些反应又受转录、翻译和环境信号的调控。为了帮助解决这些现象，各种棉花品种将根据它们对肾形线虫感染的反应来确定其特征，同时建立急需的功能基因组资源。该项目将从感染肾形线虫或根结线虫的棉花根以及经不同植物防御反应激发子处理的棉花根中开发和测序cdna和小RNA文库。这些研究将包括对肾形线虫感染反应不同的四个棉花品种。产生的序列将用于开发微阵列，然后可用于棉花-肾形线虫相互作用的功能基因组研究。在这个项目中产生的基因组数据将与棉花研究界共享，以促进开发棉花根的Uniges集合、全球基因表达工具和Silico中的比较基因组分析。EST序列将保存在http://ncbi.nlm.nih.gov.的国家生物技术信息中心所有研究成果将在项目网站http://reniform.aamu.edu.上公开发布。教育成果和资源将在项目网站和http://www.plantgdb.org/PGROP/pgrop.php.上提供更广泛的影响：雷尼形线虫是美国最重要的棉花新兴害虫，在过去十年中，其危害每年都在显着上升。本项目旨在开发资源，以了解当棉花根系感染肾形线虫时，在感病和抗病反应过程中全球基因表达的变化。由此产生的信息可以帮助遗传学家、植物育种者和生物技术专家设计有效的策略来控制肾形线虫。该项目对7-12年级至博士后级别的学生进行了外联和培训，重点是阿拉巴马州黑带地区未被充分代表的少数群体和面临风险的学校的科学教师。

PI: Ramesh Kantety (Alabama Agricultural and Mechanical University)CoPIs: Elica S. Moss (Alabama Agricultural and Mechanical University) and Katheryn S. Lawrence (Auburn University, subawardee)Collaborators: Yong-Li Xiao (J. Craig Venter Institute, Inc., subawardee) and Bruce Roe (University of Oklahoma - Norman, subawardee)Cotton is the most extensively cultivated natural fiber in the world. However cotton requires intensive management in order to control pests and pathogens. Damage to cotton caused by nematodes alone is poised to reach $1 billion in the near future; thus far there are no cotton cultivars resistant to reniform nematodes. Interaction of reniform nematodes with cotton roots elicit complex plant responses at biochemical, morphological and physiological levels which in turn are regulated by transcriptional, translational, and environmental signals. To help address such phenomena, various cotton species will be characterized for their responses to the reniform nematode infection while building much needed functional genomic resources. The project will develop and sequence cDNA and small RNA libraries from cotton roots infected with reniform or root-knot nematodes as well as from cotton roots treated with various elicitors of plant defense responses. Four cotton species which react differently to the reniform nematode infection will be included in these studies. Sequences generated will be used in the development of microarrays that can then be used in the functional genomic studies of cotton-reniform nematode interactions. The genomic data generated in this project will be shared with cotton research community to facilitate the development of unigenes sets for cotton roots, global gene expression tools, and comparative genomic analyses in silico. EST sequences will be deposited at the National Center for Biotechnology Information at http://ncbi.nlm.nih.gov. All research outcomes will be made available publicly on the project website at http://reniform.aamu.edu. Educational outcomes and resources will be available at the project website and through http://www.plantgdb.org/PGROP/pgrop.php. Broader Impacts: Reniform nematode is the most important emerging pest of cotton in the US and its damage has been significantly rising every year for the last decade. This project aims to develop resources to understand the global gene expression changes during susceptible and resistant responses of cotton roots when infected with reniform nematodes. The resulting information can aid geneticists, plant breeders and biotechnologists in devising effective strategies for the control of reniform nematodes. This project undertakes outreach and training of 7-12 graders up to post-doctoral level with a major emphasis on underrepresented minorities and science teachers from at-risk schools located in the Black Belt regions of Alabama.