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Identification of mRNAs with polar distributions in complex cells and their functional characterization

复杂细胞中极性分布 mRNA 的鉴定及其功能表征

基本信息

批准号：
181600422
负责人：
Professorin Dr. Maria Leptin, Ph.D.
金额：
--
依托单位：
Institut für Entwicklungsbiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/181600422?language=en
关键词：
Identification mRNAs polar distributions complex

项目摘要

Many cells have highly polarized morphologies that serve specialized functions. In some cell types the local synthesis of proteins from localized mRNAs contributes to polarized cell behaviour. We pro-pose here to conduct a genetic screen for mRNAs with polar distributions in different cell types which will be based on an in vivo screening method we have developed to identify mRNAs with polarized distributions in the branched cells of the Drosophila tracheal system. A pilot screen has identified genes required for cell morphogenesis that have not been found by other screening methods. The preliminary results suggest the existence of cell type specific mRNA recognition and processing sys-tems, and show that the screen provides an unbiased approach to large-scale identification of local-ized RNAs that can be applied to any cell type. The full-scale screen for localized mRNAs we propose here is therefore expected to have two long-term results. It will yield further genes for the elucidation of cell morphogenetic mechanisms and the sequences of the genes will constitute a database of mRNAs with tissue-specific polar distributions, and provide a basis for the bioinformatic prediction of localisation signals. Our database obtained from screening tracheal cells,oocytes and the follicle cell epithelium will be enriched by input from collaborating laboratories who will use the stocks we generate to screen other cell types.

许多细胞具有高度极化的形态，具有特殊的功能。在某些细胞类型中，由局部mrna合成的蛋白质有助于极化细胞行为。我们在此提议对不同细胞类型中具有极性分布的mrna进行遗传筛选，这将基于我们开发的体内筛选方法来识别果蝇气管系统分支细胞中具有极性分布的mrna。试点筛选已经确定了其他筛选方法未发现的细胞形态发生所需的基因。初步结果表明存在细胞类型特异性mRNA识别和处理系统，并表明该筛选为大规模鉴定可应用于任何细胞类型的局部化rna提供了一种公正的方法。因此，我们在这里提出的本地化mrna的全面筛选预计会有两个长期的结果。这将产生进一步阐明细胞形态发生机制的基因，这些基因的序列将构成具有组织特异性极性分布的mrna数据库，并为定位信号的生物信息学预测提供基础。我们从筛选气管细胞、卵母细胞和卵泡细胞上皮中获得的数据库将通过合作实验室的输入而丰富，合作实验室将使用我们生成的库存来筛选其他细胞类型。