FGF receptor dependent cell migration in Drosophila

果蝇中 FGF 受体依赖性细胞迁移

• 批准号: 5246212
• 负责人: Professorin Dr. Maria Leptin, Ph.D.
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5246212?language=en
• 关键词: FGF; receptor; dependent; cell; migration

The formation of the mesoderm and the branched network of the tracheae in Drosophila is associated with cell migration, changes in cell shape and cell rearrangements. The morphogenesis of both of these tissues is controlled by the FGF signal transduction pathway. This project aims to identify the mechanism by which the components of the FGF signalling pathway cause motile behaviour. The first step will be to investigate the changes that occur within mesodermal and tracheal cells upon activation of the FGF signal transduction pathway. Initially a number of markers will be used to gain a detailed description of the cytoskeleton, the polarity and the subcellular events of the cells that take part in these morphogenetic processes in wildtype embryos. In addition, the specific cell contacts made by the mesoderm as it spreads out on the ectoderm will be investigated using electron microscopy. We will then study how these events are affected when the FGF signal transduction pathway is disrupted, and how the effects differ depending on the level of the signalling pathway at which the disruption occurs. The role of Dof in particular will be investigated by examining the extent to which mutant forms of the protein can rescue the defects in morphogenesis and activation of FGF target genes that are observed in Dof mutant embryos. It is hoped that this strategy will identify parts of the protein associated with distinct functions, especially those necessary for the link to the cytoskeleton, and shed light on the mechanisms by which the FGF signal transduction pathway affects cell migration.

果蝇中胚层和气管分支网络的形成与细胞迁移、细胞形状变化和细胞重排有关。这两种组织的形态发生都受成纤维细胞生长因子信号转导途径的控制。该项目旨在确定成纤维细胞生长因子信号通路的组成部分引起运动行为的机制。第一步将是研究激活成纤维细胞生长因子信号转导通路后中胚层和气管细胞内发生的变化。最初，将使用一些标记来详细描述野生型胚胎中参与这些形态发生过程的细胞的细胞骨架、极性和亚细胞事件。此外，还将使用电子显微镜研究中胚层在外胚层上展开时所形成的特定细胞接触。然后，我们将研究当成纤维细胞生长因子信号转导通路被干扰时，这些事件是如何受到影响的，以及这些影响如何根据发生中断的信号通路的水平而不同。DOF的作用将通过检测突变形式的蛋白质在多大程度上可以挽救在DOF突变胚胎中观察到的成纤维细胞生长因子靶基因的形态发生和激活方面的缺陷来研究。人们希望这一策略将识别与不同功能相关的蛋白质部分，特别是那些与细胞骨架连接所必需的部分，并阐明成纤维细胞生长因子信号转导途径影响细胞迁移的机制。