Acyclic Terpene Utilization (Atu) in Pseudomonads

假单胞菌中的无环萜烯利用 (Atu)

• 批准号: 18242844
• 负责人: Professor Dr. Dieter Jendrossek
• 金额: --
• 依托单位: Institut für Mikrobiologie (IMB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/18242844?language=en
• 关键词: Acyclic; Terpene; Utilization; Atu; Pseudomonads

Acyclic monoterpenes such as citronellol and geraniol are aroma compounds that are used in perfume and in food industry. This is astonishing because several reports indicate strong biological effects of acyclic monoterpenes as signalling substances, insecticides and cytotoxic compounds (apoptosis inducers). Metbolism of citronellol and related methyl-branched molecules is hardly understood and recent literature data on catabolism of citronellol in Pseudomonas aeruginosa (Diaz-Perez et al. [2004] AEM 70:5102) are in contradiction to findings in our lab. The aim of this proposal is to unravel the function of two gene clusters of P. aeruginosa (PA2011-PA2016 and PA2886-PA2893) with respect to the citronellol pathway (upper pathway) and to leucine degrading pathway (lower pathway); in particular, the respective key enzymes (geranyl-CoA carboylase and methyl-crotonyl-CoA carboxylase) and 3-hydroxy-3-isohexenyl-glutaryl CoA acetate lyase have to be identified and assigned to the corresponding genes. The planned investigations include identification and analysis of (at least one) molybdenum-dependent step in geraniol oxidation that is surprisingly different in citronellol oxidation.

无环单萜如香茅醇和香叶醇是用于香料和食品工业的芳香化合物。这是令人惊讶的，因为一些报告表明，无环单萜作为信号物质，杀虫剂和细胞毒性化合物（细胞凋亡诱导剂）的强烈生物效应。香茅醇和相关甲基分支分子的代谢作用几乎不被理解，并且最近关于铜绿假单胞菌中香茅醇的催化剂的文献数据（Diaz-Perez等人[2004] AEM 70：5102）与我们实验室的发现相矛盾。本研究的目的是阐明铜绿假单胞菌两个基因簇的功能（PA 2011-PA 2016和PA 2886-PA 2893）（上途径）和亮氨酸降解途径（下通道）;特别是，各自的关键酶（香叶酰-CoA羧化酶和甲基-巴豆酰-CoA羧化酶）和3-羟基-3-异己烯基-戊二酰-CoA乙酸裂解酶必须被鉴定并分配到相应的基因。计划的研究包括鉴定和分析香叶醇氧化中的（至少一个）与香茅醇氧化惊人不同的氘依赖性步骤。